Escherichia coli (Migula) Castellani and Chalmers (ATCC® 33572)

Strain Designations: LE392 [NRRL B-14219]  /  Type Strain: no  /  Biosafety Level: 1

Classification Enterobacteriaceae, Escherichia
Strain Designations LE392 [NRRL B-14219]
Application
transformation host
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Genotype F- supE44 supF58 lacY1 or deltalac(I-Y)6 galK2 galT22 metB1 trpR55 hsdR514 mcrB lambda-
Type Strain no
Comments
A host for plating lambda libraries or clones requiring suppression of amber mutations (i.e. Charon 4A, Charon 21A, etc.). Strain LE392 same as NRRL B-14219.
Medium ATCC® Medium 1065: LB Agar/Broth, Miller
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Genotype F- supE44 supF58 lacY1 or deltalac(I-Y)6 galK2 galT22 metB1 trpR55 hsdR514 mcrB lambda-
Name of Depositor P Leder
References

Murray NE, et al. Lambdoid phages that simplify the recovery of in vitro recombinants. Mol. Gen. Genet. 150: 53-61, 1977. PubMed: 319344

Leder P, et al. EK2 derivatives of bacteriophage lambda useful in the cloning of DNA from higher-organisms: the lambdagtWES system. Science 196: 175-177, 1977. PubMed: 322278

Brunel F, et al. Cloning of bacteriophage T5 DNA fragments in plasmid pBR322 and bacteriophage lambdagtWES. Gene 8: 53-68, 1979. PubMed: 535730

Gowda K, et al. Protein SRP54 of human signal recognition particle: cloning, expression, and comparative analysis of functional sites. Gene 207: 197-207, 1998. PubMed: 9511762