Escherichia coli (Migula) Castellani and Chalmers (ATCC® 29181)

Strain Designations: K-12 strain S 4362  /  Type Strain: no  /  Biosafety Level: 1

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Classification Enterobacteriaceae, Escherichia
Strain Designations K-12 strain S 4362
Susceptibility testing sulfonamide
Susceptibility testing of sulfonamides in the presence of trimethoprim
Derived from existing strain
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Type Strain no
Antibiotic Resistance
Resistant to trimethoprim and streptomycin (R factor 483) but not sulfonamides
Medium ATCC® Medium 3: Nutrient agar or nutrient broth
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Name of Depositor Wellcome Research Laboratories
Derived from existing strain

Hedges RW, et al. R Factors conferring resistance to trimethoprim but not solphonamides. J. Gen. Microbiol. 73: 573-575, 1972. PubMed: 4571517

Fleming MP. Trimethoprim-resistance and its transferability in E. coli isolated from calves treated with trimethoprim-sulphadiazine: a two year study. J. Hyg. 71: 669-677, 1973.

Derived by mating a K-12 strain with an R-factor-carrying Escherichia coli

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation