MPC 862L
CRL-3540 ™
CRL-3540 ™
ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Pheochromocytoma research
Neuroendocrine research
NF1 LOH Research
High-throughput screening
Phenylethanolamine-N-methyltransferase ·
Transcription Studies
RET signaling StudiesCRL-3540 is a pheochromocytoma developed from heterozygous Nf1 knockout mice. This cell line was established from irradiated 10 day old pups of a F1 male from 129SV (Nf1+/Nf1n31) male crossed with wild-type C57BL/6 females. CRL-3538 offers an alternative model to PC12 cells (CRL-1721). CRL-3538 differs from CRL-1721 as they show little or no expression of the NGF receptor TrkA and do not respond to NGF. (Powers et al., 2000).
The cells will produce phenyl-L-methly transferase (PNMT)messenger RNA and low levels of protein when 10 uM dexamethasone is added to culture per the cell line depositor. The cells express a high amount of Ret tyrosine kinase receptor.
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
Gibco™ Recovery™ Cell Culture Freezing Media (ThermoFisher Scientific catalog # 12648-010).
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