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hNET lambda1

99548

Organism
Homo sapiens
Clone type
Clone
Applications
Molecular biology
Product format
Freeze-dried
Shipping information
bacteria-free lysate
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Biosafety Icon BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): NotI-- >9.0; EcoRI-- >9.0, 5.2, 0.6, 0.5; SacI-- >9.0, 4.4, 3.2, 2.6, 2.0, 1.7, 0.25; XbaI-- >9.0, 4.0, 1.2.
The insert contains the following restriction sites (approximate kb from 5' end): EcoRI--1.30, 1.90, 2.40, 7.60; SacI--3.35, 7.75, 7.95, 9.90, 13.05; XbaI--9.55, 13.45.
ATCC 99548 contains exons 6-14 of the 14 exons which comprise the norepinephrine transporter gene.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
42.70000076293945
Intact vector size
41.900
Vector name
lambdaFIX
Type of vector
phage
Host range
Escherichia coli
Vector end
XhoI
Cloning sites
SalI; XhoI; EcoRI
Markers
ampR
Polylinker sites
XbaI; SacI; NotI; SacI; SalI; XhoI; EcoRI; XbaI
Promoters
T7; T3
Replicon
lambda

Insert information

Insert size (kb)
13.5
Type of DNA
genomic
Insert source
lung fibroblast
Insert tissue
lung fibroblast
Insert information
DESCRIPTION OF INSERT COMPONENT:
norepinephrine), member 5
ORF's seq. position: GDB probe: GDB:136379 ()
Cross references: DNA Seq. Acc.: X91118
Nucleotides ~7390~8410 of the insert correspond to
nucleotides 1-1017 of X91118.
DNA Seq. Acc.: X91119
Nucleotides ~9600~10400 of the insert correspond to
nucleotides 1-922 of X91119.
DNA Seq. Acc.: X91124
Nucleotides ~1700~2060 of the insert correspond to
nucleotides 1-357 of X91124.
DNA Seq. Acc.: X91125
Nucleotides ~3110~3520 of the insert correspond to
nucleotides 1-361 of X91125.
DNA Seq. Acc.: X91126
Nucleotides ~4060~4460 of the insert correspond to
nucleotides 1-395 of X91126.
DNA Seq. Acc.: X91127
Nucleotides ~5780~6760 of the insert correspond to
nucleotides 1-980 of X91127.
Genome
Homo sapiens
Chromosome
16
16 q13-q21
Gene name
solute carrier family 6 (neurotransmitter transporter,
Gene product
solute carrier family 6 (neurotransmitter transporter, norepinephrine), member 5 [NET1]
Gene symbol
SLC6A5; NET1
Contains complete coding sequence
No
Insert end
Sau3AI

Handling information

Temperature
37°C
Handling notes
Restriction digests of the clone give the following sizes (kb): NotI-- >9.0;
EcoRI-- >9.0, 5.2, 0.6, 0.5; SacI-- >9.0, 4.4, 3.2, 2.6, 2.0, 1.7, 0.25; XbaI--
>9.0, 4.0, 1.2.
- ATCC staff

The insert contains the following restriction sites (approximate kb from 5'
end): EcoRI--1.30, 1.90, 2.40, 7.60; SacI--3.35, 7.75, 7.95, 9.90, 13.05;
XbaI--9.55, 13.45.
- personal communication

ATCC 99548 contains exons 6-14 of the 14 exons which comprise the norepinephrine
transporter gene.
- Biochem. Biophys. Res. Commun. 215: 1145-1150, 1995

History

Depositors
P Porzgen
Cross references
GenBank X91118
GenBank X91119
GenBank X91124
GenBank X91125
GenBank X91126
GenBank X91127
GenBank 774704

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Porzgen P, et al. Molecular cloning and organization of the coding region of the human norepinephrine transporter gene. Biochem. Biophys. Res. Commun. 215: 1145-1150, 1995. PubMed: 7488042

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