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Enterococcus hirae Farrow and Collins


Type strain
Product category
Strain designation
R [NCIB 8191]
Type strain
Food testing
Product format
Storage conditions
2°C to 8°C


ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information


Specific applications
Assay of arginine L-arginine
Assay of histidine L-histidine
Assay of isoleucine L-isoleucine
Assay of leucine
Assay of threonine
Assay of tryptophan L-tryptophan
Assay of valine L-valine
Food testing


ATCC 9790 and ATCC 8043 are the same.
Nucleoid condensation and meosome growth
Cell wall composition

Handling information

Handling procedure
  1. Open vial according to enclosed instructions or visit for instructions.
  2. Rehydrate the entire pellet with approximately 0.5 mL of #44 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #44 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary tubes.
  3. Use several drops of the primary broth tube to inoculate a #44 plate and/or #44 agar slant.
  4. Incubate at 37°C for 24 hours.
Handling notes
This strain is a member of Lancefield’s Group D.
Additional information on this culture is available on the ATCC® web site at  


Deposited as
Streptococcus lactis (Lister) Lohnis
Merck & Co., Inc.
Cross references
GenBank AX109952 Sequence 685 from Patent WO0123604.
GenBank U22540 Enterococcus hirae insertion sequence.
GenBank U22541 Enterococcus hirae insertion sequence.
GenBank X63285 E.hirae gene nakA for Na+-Atpase 73kDa subunit.
GenBank L00924 Enterococcus hirae ribosomal RNA gene sequences.
GenBank D16334 E. hirae DNA for Na+-ATPase K subunit, complete cds.
GenBank X68646 E.hirae psr gene for penicillin binding protein repressor.
GenBank L00925 Enterococcus hirae transfer RNA and ribosomal RNA gene sequences.
GenBank D13816 E. hirae DNA for Na+ -ATPase alpha and beta subunits, complete cds.
GenBank D17462 Enterococcus hirae ntp genes for Na+ -ATPase subunits, complete cds.
GenBank U42211 Enterococcus hirae PBP 5 synthesis repressor (psr) gene, complete cds.
GenBank M64265 Streptococcus faecalis F1F0 ATPase (atpE, atpF, atpH and atpA) genes, complete cds.
GenBank M77639 Enterococcus hirae beta-1,4-N-acetylmuramoylhydrolase (muramidase-2) gene, complete cds.
GenBank AJ302066 Enterococcus hirae prfA gene for penicillin binding protein related factor A and ponA gene for penicillin binding protein 1A.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

Frequently Asked Questions


Curated Citations

Massidda O, et al. Evidence that the PBP 5 synthesis repressor (psr) of Enterococcus hirae is also involved in the regulation of cell wall composition and other cell wall-related properties. J. Bacteriol. 178: 5272-5278, 1996. PubMed: 8752348

AOAC International Amino acids in vitamin preparations. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 960.47.

Farrow JA, Collins MD. Enterococcus hirae, a new species that includes amino acid assay strain NCDO 1258 and strains causing growth depression in young chickens. Int. J. Syst. Bacteriol. 35: 73-75, 1985.

Tsien HC, et al. Structural arrangement of polymers within the wall of Streptococcus faecalis. J. Bacteriol. 133: 372-386, 1978. PubMed: 412837

Joseph R, Shockman GD. Autolytic formation of protoplasts (autoplasts) of Streptococcus faecalis; location of active and latent autolysin. J. Bacteriol. 127: 1482-1493, 1976. PubMed: 821932

View All Curated Citations for this Product

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