Saccharomyces paradoxus Bachinskaya
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
1. Open an ampoule according to enclosed instructions.
2. From a single test tube of sterile distilled water (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a sterile pipette and apply directly to the pellet. Stir to form a suspension.
3. Aseptically transfer the suspension back into the test tube of sterile distilled water.
4. Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; longer (e.g., overnight) rehydration might increase viability of some fungi..
5. Mix the suspension well. Use several drops (or make dilutions if desired) to inoculate recommended solid or liquid medium. Include a control that receives no inoculum.
6. Incubate the inoculum at the propagation conditions recommended.
7. Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 1-2 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
- Allozyme analysis.
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Naumov GI, et al. Differentiation of European and Far East Asian populations of Saccharomyces paradoxus by allozyme analysis. Int. J. Syst. Bacteriol. 47: 341-344, 1997. PubMed: 9103619
Naumova ES, et al. Genetic variation among European strains of Saccharomyces paradoxus: results from DNA fingerprinting. Syst. Appl. Microbiol. 23: 86-92, 2000. PubMed: 10879982