Aspergillus brasiliensis Varga et al.
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Recommended as a test microorganism for ASTM C1338: Standard Test Method for Determining Fungi Resistance of Insulation Materials and Facings
Recommended as a test microorganism for ASTM D2020: Standard Test Methods for Mildew (Fungus) Resistance of Paper and Paperboard
Recommended as a test microorganism for ASTM D4300: Standard Test Methods for Ability of Adhesive Films to Support or Resist the Growth of Fungi
Recommended as a test microorganism for ASTM G21: Standard Practice for Determining Resistance of Synthetic Polymeric Materials to Fungi
For freeze-dry (lyophilized) ampoules:
Colonies initially white, mycelium growing rapidly (to cover a plate in 8 to 10 days), soon producing dense layer of erect smooth-stiped, thick-walled conidiophores terminated by globose vesicles bearing phialides (uniseriate) or (commonly) metulae with phialides (biseriate) which produce dry chains of conidia. Reverse of plate pale yellow or cream, often showing radiating ridges in mycelium. Spore heads radiate, sometimes dividing into columns with age, initially pale, becoming dark brown to black. Individual conidia spherical, mid-to-dark brown, highly roughened with ridges and blunt or pointed protuberances, 3.5 to 6 µm in diameter.
Will grow equally well up to at least 37°C. Sporulation may be inhibited in plates sealed completely with tape or film. Colonies grown directly from rehydrated spores may exhibit sectoring, with areas of varying levels of sporulation.
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Sakano Y, et al. Pullulan 4-glucanohydrolase from Aspergillus niger. Arch. Biochem. Biophys. 153: 180-187, 1972. PubMed: 4650606
Inoue Y, et al. Metabolism of 2-ketoaldehydes in mold: purification and characterization of glyoxalase I from Aspergillus niger. J. Biochem. 102: 583-589, 1987. PubMed: 3123469
ASTM International Standard Test Methods for Ability of Adhesive Films to Support or Resist the Growth of Fungi. West Conshohocken, PA:ASTM International;ASTM Standard Test Method D 4300-01.
ASTM International Standard Test Methods for Resistance of Adhesive Preparations in Container to Attack by Bacteria, Yeast, and Fungi. West Conshohocken, PA:ASTM International;ASTM Standard Test Method D 4783-01e1.
ASTM International Standard Practice for Determining Resistance of Synthetic Polymeric Materials to Fungi. West Conshohocken, PA:ASTM International;ASTM Standard Test Method G 0021-96 (Reapproved 2002).