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Pseudomonas paraeruginosa Rudra et al.

9027

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Pseudomonas paraeruginosa strain R. Hugh 813 was isolated from an outer ear infection. This whole-genome sequenced culture can be used in media testing and efficacy testing, and it is recommended as a test culture in USP chapters <51>, <60>, <61>, <62>, and <71>.
Product category
Bacteria
Classification
Bacteria, Pseudomonadota, Gammaproteobacteria, Pseudomonadales, Pseudomonadaceae, Pseudomonas, Pseudomonas aeruginosa group
Strain designation
R. Hugh 813
Type strain
Yes
Genome sequenced strain
Yes
Former name
Pseudomonas aeruginosa (Schroeter) Migula
Isolation source
Outer ear infection
Applications
Bioinformatics
Media testing
Quality control
Pharmaceutical testing
Water testing
Product format
Freeze-dried
Storage conditions
2°C to 8°C
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
Assay of antimicrobial preservatives
Efficacy testing
Media testing
Preparatory test control
Quality control strain
Sterility testing
Produces rhamnolipid surfactant
Pharmaceutical and Personal Care
Recommended as a test microorganism for EP 2.6.1 Sterility
Recommended as a test microorganism for EP 2.6.12 Microbial Examination of Non-Sterile Products: Microbial Enumeration Tests
Recommended as a test microorganism for EP 2.6.27 Microbiological Control of Cellular Products
Recommended as a test microorganism for EP 2.6.31 Microbiological Examination of Herbal Medicinal Products for Oral Use
Recommended as a test microorganism for EP 5.1.3 Efficacy of Antimicrobial Preservation
Recommended as a test microorganism for EP Monographs
Recommended as a test microorganism for JP 16 4.05 I Microbiological Examination of Non-sterile Products: Total Viable Aerobic Count
Recommended as a test microorganism for JP 16 4.05 II Microbiological Examination of Non-sterile Products: Tests for Specificed Micro-organisms
Recommended as a test microorganism for JP 16 4.06 Sterility Test
Recommended as a test microorganism for JP 16 G4 Microorganisms: Preservatives-Effectiveness Tests
Recommended as a test microorganism for JP 16 G8 Quality Control of Water for Pharmaceutical Use
Recommended as a test microorganism for USP <51> Antimicrobial Effectivness Testing
Recommended as a test microorganism for USP <60> Microbiological Examination of Nonsterile Products: Tests for Burkholderia cepacia Complex
Recommended as a test microorganism for USP <61> Microbiological Examination of Nonsterile Products: Microbial Enumeration Tests
Recommended as a test microorganism for USP <62> Microbiological Examination of Nonsterile Products: Tests for Specified Microorganisms
Recommended as a test microorganism for USP <71> Sterility Tests

Recommended as a test microorganism for ASTM E2315: Standard Guide for Assessment of Antimicrobial Activity Using a Time-Kill Procedure

Recommended as a test microorganism for ASTM E640: Standard Test Method for Preservatives in Water-Containing Cosmetics

Recommended as a test microorganism for ISO 11930: Cosmetics — Microbiology — Evaluation of the antimicrobial protection of a cosmetic product

Preceptrol
No

Characteristics

Comments

Component of ATCC MSA-1002, ATCC MSA-1003, and ATCC MSA-2002.

The name of Pseudomonas aeruginosa strain R. Hugh 813 (ATCC 9027) was changed to Pseudomonas paraeruginosa. This change is in response to phylogenomic and comparative genomic studies performed by Rudra et al. that identified two distinct clades of P. aeruginosa. Strains in Clade-2 were transferred to the novel species P. paraeruginosa with ATCC 9027 designated as the type strain.

Handling information

Medium
Temperature
37°C
Atmosphere
Aerobic
Handling procedure
  1. Open vial according to enclosed instructions.
  2. Using a single tube of #3 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette. Rehydrate the entire pellet.
  3. Aseptically transfer this aliquot back into the broth tube. Mix well.
  4. Use several drops of the suspension to inoculate a #3 agar slant and/or plate.
  5. Incubate all tubes and plate at 37°C for 24 hours.
Handling notes
Both fluorescein and pyocyanin pigments are produced.
Purified genomic DNA of this strain is available (ATCC 9027D-5, ATCC 9027DQ). This strain is available as Certified Reference Material (ATCC CRM-9027).
Additional information on this culture is available on the ATCC web site at www.atcc.org.

Quality control specifications

Verification method
Whole-genome Sequencing

History

Deposited as
Pseudomonas aeruginosa (Schroeter) Migula
Depositors
CP Hegarty
Cross references
GenBank JN188339.1 Pseudomonas aeruginosa strain ATCC 9027 MerP gene, complete cds
GenBank JF927784.1 Pseudomonas aeruginosa strain ATCC 9027 mercuric ion transporter (merT) gene, complete cds
GenBank HM060302.1 Pseudomonas aeruginosa putative nitrite reductase (nirS) gene, partial cds
GenBank FM946027.1 Pseudomonas aeruginosa partial rhlB gene for rhamnosyl transferase 2, strain ATCC 9027
GenBank FM946026.1 Pseudomonas aeruginosa partial rhlA gene for rhamnosyl transferase 1, strain ATCC 9027
GenBank EF127873.1 Pseudomonas aeruginosa MucD (mucD) gene, complete cds

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

ASTM International Standard Test Method for Preservatives in Water-Containing Cosmetics. West Conshohocken, PA

Zhang Y, Miller RM. Enhanced octadecane dispersion and biodegradation by a Pseudomonas rhamnolipid surfactant (biosurfactant). Appl. Environ. Microbiol. 58: 3276-3282, 1992. PubMed: 1444363

British Pharmacopoeia Commission Test for sterility. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI A, 2003

British Pharmacopoeia Commission Tests for microbial contamination. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI B, 2003

British Pharmacopoeia Commission Efficacy of antimicrobial preservation. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI C, 2003

View All Curated Citations for this Product

Frequently Asked Questions

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