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Bacteroides vulgatus Eggerth and Gagnon

8482

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This is a type strain that has applications in media and sterility testing.  This organism is included in the bioMerieux VITEK 2 - ANC Comprehensive and Traditional Quality Control Organism Sets.
Product category
Bacteria
Product type
Anaerobe
Strain designation
[NCTC 11154]
Type strain
Yes
Genome sequenced strain
Yes
Applications
Bioinformatics
Media testing
Quality control
Product format
Frozen
Storage conditions
-80°C or colder
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Price: $259.00 EA
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Documentation

Biosafety Icon Biosafety Level 2

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
Media testing
Quality control strain
Sterility testing
Pharmaceutical and Personal Care
Preceptrol
No

Characteristics

Comments
Genome sequenced strain

This organism is included in the bioMerieux VITEK 2 ANC Comprehensive and Traditional Quality Control Organism Sets

Component of MSA-1002, MSA-1003, MSA-1006, MSA-2002, and MSA-2006.

Handling information

Medium
Temperature
37°C
Atmosphere
Anaerobic
Handling procedure

  1. Open thawed vial according to enclosed instructions or visit www.atcc.org for instructions.
  2. Under anaerobic conditions, aseptically transfer the entire contents to a 5-6 mL tube of #2107 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary broth tubes. Best practice dictates the use of pre-reduced media.
  3. Use several drops of the primary broth tube to inoculate a brucella blood plate plate and/or #260 agar slant.
  4. Incubate in an anaerobic atmosphere at 37°C for 24 hours. Incubate one agar plate aerobically at 37°C to check for contamination.

ANAEROBIC CONDITIONS:
Anaerobic conditions for transfer may be obtained by the use of an anaerobic gas chamber or placement of test tubes under a gassing cannula system connected to anaerobic gas.

Anaerobic conditions for incubation may be obtained by any of the following:
  • Loose screw caps on test tubes in an anaerobic chamber
  • Loose screw caps on test tubes in an activated anaerobic gas pack jar
  • Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained
Handling notes

Media #1490 is an acceptable alternative media for this item.

This item may take 48 hours to recover from preservation

Anaerobe Systems Brucella Blood plate (AS-111 or AS-141) can be used to analyze colony morphology and purity.

Always use freshly prepared pre-reduced media or pre-reduced media that has been previously prepared but stored under anaerobic conditions. Resazurin in the media is a color indicator for anaerobic conditions.

Observance of pink color in medium before use or during incubation shows anaerobic conditions have not been met and oxidation has occurred. Medium should be discarded.

Purified genomic DNA of this strain is available as ATCC® 8482D-5™.

Additional information on this culture is available on the ATCC® web site at www.atcc.org.

Quality control specifications

Verification method
Whole-genome Sequencing

History

Deposited as
Bacteroides vulgatus Eggerth and Gagnon
Depositors
AH Eggerth
Chain of custody
ATCC <-- A H Eggerth
Cross references
GenBank M58762 Bacteroides vulgatus 16S ribosomal RNA.
GenBank AJ279041 Bacteroides vulgatus partial gyrA gene for DNA gyrase subunit A, strain ATCC 8482.
GenBank CP000139 Bacteroides vulgatus ATCC 8482, complete genome

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Moore WE, Holdeman LV. New names and combinations in the genera Bacteroides Castellani and Chalmers, Fusobacterium Knorr, Eubacterium Prevot, Propionibacterium Delwich, and Lactobacillus Orla-Jensen. Int. J. Syst. Bacteriol. 23: 69-74, 1973.

Cato EP, Johnson JL. Reinstatement of species rank for Bacteroides fragilis, B. ovatus, B. distasonis, B. thetaiotaomicron, and B. vulgatus: Designation of neotype strains for Bacteroides fragilis (Veillon and Zuber) Castellani and Chalmers and Bacteroides thetaiotaomicron (Distaso) Castellani and Chalmers. Int. J. Syst. Bacteriol. 26: 230-237, 1976.

Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard - 3rd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M22-A3.

US Food & Drug Administration. GENERAL BIOLOGICAL PRODUCTS STANDARDS; General Provisions; Sterility Code of Federal Regulations Title 21: 21CFR610.12, Subpart B, 2005

Wang RF, et al. PCR detection and quantitiation of predominant anaerobic bacteria in human and animal fecal samples. Appl. Environ. Microbiol. 62: 1242-1247, 1996. PubMed: 8919784

View All Curated Citations for this Product

Frequently Asked Questions

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