Lactobacillus rhamnosus (Hansen) Collins et al.


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Type strain. Used for assay of folic acid and riboflavin. Produces folate transport protein. Deposited as Lactobacillus casei epsilon; later L. helveticus.
Product category
Product type
Preceptrol culture
Strain designation
[BUCSAV 227, M. Rogosa V300, M.E. Sharpe H2, NCDO 243, NCIB 6375, NCIB 8010, NCTC 6375, NRC 488, P.A. Hansen 300, R.P. Tittsler 300]
Type strain
Genome sequenced strain
Food testing
Product format
Storage conditions
2°C to 8°C
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information


Specific applications
Assay of folic acid
Assay of niacinamide nicotinamide
Assay of riboflavin vitamin B2
Produces folate transport protein
Food testing


Respiratory physiology
Extraction of acidic metabolites
Pyruvate utilization
Technical information
ATCC Technical Services does not have technical information on patent deposits that are not produced or characterized by ATCC. Additional information can be found in the corresponding patent available from the patent holder or with the U.S. and/or international patent office.

Handling information

95% Air, 5% CO2
Handling procedure
  1. Open vial according to enclosed instructions.
  2. From a single tube of #416 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette and use to rehydrate the pellet.
  3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.
  4. Use several drops of this suspension to inoculate a second tube of broth, a slant and/or a plate.
  5. Incubate tubes and plate at 37°C in an atmosphere of 5% CO2 for 24 to 48 hours. Loosen screw caps of all test tubes during the incubation period.
Handling notes
Additional information on this culture is available on the ATCC® web site at

Quality control specifications

Verification method
Whole-genome Sequencing


Deposited as
Lactobacillus casei (Orla-Jensen) Holland
FM Strong
Patent depository
This material was deposited with the ATCC Patent Depository to fulfill U.S. or international patent requirements. This material may not have been produced or characterized by ATCC.  As an International Depository Authority (IDA) for patent deposits, ATCC is required to complete viability testing only at time of initial deposit of patent material. Patent deposits are made available on behalf of the Depositor when the pertinent U.S. or international patent is issued, but material may not be used to infringe the patent claims.
Cross references
GenBank AF192553 Lactobacillus rhamnosus dlt operon, complete sequence.
GenBank L08854 Lactobacillus casei valyl-tRNA synthetase gene, complete cds.
GenBank J05221 L.carei folylpoly-gamma-glutamate synthetase gene, complete cds.
GenBank AF323539 Lactobacillus rhamnosus strain ATCC 7469 glycosyltransferase gene, partial cds.
GenBank AF323528 Lactobacillus rhamnosus strain ATCC 7469 priming glycosyltransferase gene, partial cds.
GenBank U43894 Lactobacillus casei dlt operon DltA, DltB, and DltC genes, complete cds, and DltD gene, partial cds.
GenBank M83993 Lactobacillus casei D-alanine-activating enzyme gene, complete cds and a potential basic membrane protein (involved in D-ala-LTA biosynthesis) gene, 5' end.
GenBank AF015453 Lactobacillus rhamnosus 6-phospho-beta-glucosidase homolog gene, partial cds; GNTR transcriptional regulator homolog and surface located protein genes, complete cds.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


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While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

This material is cited in a US and/or international patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Depositor of the party to which the material was furnished.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.



Curated Citations

Henderson GB, et al. Kinetic evidence for two interconvertible forms of the folate transport protein from Lactobacillus casei. J. Bacteriol. 163: 1147-1152, 1985. PubMed: 3928596

Colwell RR, Liston J. Taxonomic relationships among the pseudomonads. J. Bacteriol. 82: 1-14, 1961. PubMed: 13694873

Biochem. J. 69: 403, 1958.

Biochim. Biophys. Acta 54: 614, 1961.

Biochim. Biophys. Acta 59: 273, 1962.

View All Curated Citations for this Product

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