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Thermococcus zilligii Ronimus et al.


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Product category
Product type
Drug-resistant bacterium
Strain designation
DSM 2770 [AN1, ANI, JCM 10554, TG 57]
Type strain
Genome sequenced strain
Isolation source
Anaerobic mud from alkaline terrestrial freshwater hot spring in Kuirau Park
Geographical isolation
New Zealand; North Island; Rotorua
Antimicrobial resistance research
Drug development
Product format
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information


Specific applications


Extremophile type
Sulfate reducer
Susceptibility profile
Growth is inhibited by mevinolin and monensin

Handling information

Handling procedure
1. Open vial according to enclosed instructions.

2. Under anaerobic conditions, withdraw approximately 0.5 to 1.0 ml from a tube of medium #2094 with a Pasteur or 1.0 ml pipette. Re-hydrate the pellet.

3. Aseptically transfer this aliquot back into the broth tube.  Mix well.

4. After 4 to 7 days of incubation at 75oC, turbidity will be observed.

5. Transfers can now be made to other tubes of broth, slants, and/or plates

6. The aerobic plates may have a thin film in the area of heaviest inoculation; however, it should show no other signs of aerobic contamination.


Anaerobic conditions for transfer may be obtained by either of the following:

·         Use of an anaerobic gas chamber, or     

·         Placement of test tubes under a gassing cannula system hooked to anaerobic gas.   

Anaerobic conditions for incubation may be obtained by any of the following:

·         Loose screw caps on test tubes in anaerobic chamber,                        

·         Loose screw caps on test tubes in an activated anaerobic gas pack jar, or   

·         Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.  

Handling notes


          The cells are irregular cocci that grow well at pH 6.0.  The optimal temperature for growth is between 70-85°C but growth is also observed at 95°C.  Strain produces high amounts of gas, so make sure tubes are vented.  The ATCC recommends an anaerobic technique that utilizes Hungate tubes, because growth is enhanced when tubes are pressurized (20-40 psi).

          Additional information on this culture is available on the ATCC web site at

Quality control specifications

Verification method
Whole-genome Sequencing


Deposited as
Thermococcus zilligii Ronimus et al.
HW Morgan
Type of isolate
Year of origin
Cross references
GenBank U76534 Thermococcus sp. AN1 16S ribosomal RNA gene, partial sequence.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


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While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

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Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.



Curated Citations

Ronimus RS, et al. The phylogenetic position of the Thermococcus isolate AN1 based on 16S rRNA gene sequence analysis: a proposal that AN1 represents a new species, Thermococcus zilligii sp. nov.. Arch. Microbiol. 168: 245-248, 1997. PubMed: 9382704

Klages KU, Morgan HW. Characterization of an extremely thermophilic sulphur-metabolizing archaebacterium belonging to the Thermococcales. Arch. Microbiol. 162: 261-266, 1994.

Validation list no. 70. Int. J. Syst. Bacteriol. 49: 935-936, 1999.

type strain

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