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K3-1.1

63044

Organism
Mus musculus, mouse
Classification
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
Clone type
Clone
Applications
Molecular biology
Product format
Freeze-dried
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

Characteristics

Comments
The insert contains the following sites separated by (bp): EcoRI- 548- Asp700I- 950- PvuII- 147- HindIII- 547- PvuII- 640- PvuII- 145- Asp700I- 54- EcoRI.
Restriction digests of the clone give the following sizes (kb): BamHI--5.8; EcoRI--3.05, 2.95; HindIII--4.3, 1.65; PstI--3.3, 1.85, 0.6; SmaI--5.8.
Clones K4-1.1 (ATCC 63043) and K3-1.1 (ATCC 63044) differ in the 5' end of the insert. Nt 638 of the K3-1.1 insert equals nt 132 of the K4-1.1 insert.
The insert has an open reading frame of 1593 nt, 552 nt of 5' untranslated sequence and 851 nt of 3' untranslated sequence. It has an atypical N-terminal sequence and may represent an alternative splicing product.
The overall protein structure is quite similar to human ICAM1 and there is 50% homology at the amino acid level.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
5.900000095367432

Insert information

Insert size (kb)
3.0299999999999998
Type of DNA
cDNA
Insert source
myeloma NS-1 cell line
Insert tissue
myeloma NS-1 cell line
Gene product
intercellular adhesion molecule 1 [Ly47]

Handling information

History

Depositors
F Takei, KJ Horley

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Horley KJ, et al. Molecular cloning of murine intercellular adhesion molecule (ICAM-1). EMBO J. 8: 2889-2896, 1989. PubMed: 2573511

Fumio Takei, personal communication

Frequently Asked Questions

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Telephone

Telephone

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Outside the US
+1-703-365-2700
hours

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