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Scheffersomyces stipitis (Pignal) Kurtzman et Suzuki


An ampoule containing viable cells (may include spores and mycelia) suspended in cryoprotectant.
Product category
Product type
Strain designation
CBS 6054 [CCRC 21777, IFO 10063, NRRL Y-11545]
Type strain
Genome sequenced strain
Biofuel production
Industrial biotechnology
Food production research
Product format
Storage conditions
2°C to 8°C
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information


Specific applications
Bioethanol production

Potential biofuel production agent

Non-conventional yeasts for bioconversion and precision fermentation



On YM medium at 25°C after 2 days, colonies creamy white to dingy white, smooth, slightly raised, margin entire. Cells subglobose to ellipsoid, smooth, guttulate, 4-8 X 3-5 µm.
Use of corn steep liquor in fermentation of D-xylose
Genome sequencing strain (completed by the Joint Genome Institute at the Department of Energy, USA).

Handling information

Handling procedure
For freeze-dry (lyophilized) ampoules:
  1. Open an ampoule according to enclosed instructions.
  2. From a single test tube of sterile distilled water (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a sterile pipette and apply directly to the pellet.  Stir to form a suspension.
  3. Aseptically transfer the suspension back into the test tube of sterile distilled water.
  4. Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; longer (e.g., overnight) rehydration might increase viability of some fungi.
  5. Mix the suspension well.  Use several drops (or make dilutions if desired) to inoculate recommended solid or liquid medium. Include a control that receives no inoculum.
  6. Incubate the inoculum at the propagation conditions recommended.
  7. Inspect for growth of the inoculum/strain regularly. Viability is typically noticeable after 2-3 days of incubation. However, the time necessary for significant growth will vary from strain to strain.  


Handling notes
The species is also known as Pichia stipitis.
Additional, updated information on this product may be available on the ATCC® web site at

Quality control specifications

Sequenced data
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence


D1D2 region of the 26S ribosomal RNA gene



Deposited as
Pichia stipitis Pignal
Pichia stipitis Pignal; Yamadazyma stipite (Pignal) Billon-Grand; Yamadazyma stipitis (Pignal) Billon-Grand
Chain of custody
ATCC <-- CBS <-- J Santa Maria
Cross references
GenBank KU729088 ITS including 5.8S rRNA gene
GenBank KU729191 D1/D2 region of 28S rRNA gene
GenBank CM000437 Pichia stipitis CBS 6054 chromosome 1, whole genome shotgun sequence.
GenBank AAVQ00000000 Scheffersomyces stipitis CBS 6054 chromosome 1, whole genome shotgun sequencing project
GenBank AF030426 nucleotide sequence of PsCYC1
GenBank U08628 Pichia stipitis CBS 6054 ATCC 58785 autonomous replication
GenBank U08629 Pichia stipitis CBS 6054 ATCC 58785 orotidine-5'-phosphate
GenBank AF127801 Pichia stipitis xylitol dehydrogenase (XYL2) gene, complete cds.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.


Frequently Asked Questions


Curated Citations

Toivola A, et al. Alcoholic fermentation of D-xylose by yeasts. Appl. Environ. Microbiol. 47: 1221-1223, 1984.

Deed RW, et al. Xylitol production by recombinant Saccharomyces cerevisiae. Bio-Technology 9: 1090-1095, 1991. PubMed: 1367625

Amartey S, Jeffries TW. Comparison of corn steep liquor with other nutrients in the fermentation of D-Xylose by Pichia stipitis CBS 6054. Biotechnol. Lett. 16: 211-214, 1994.

Shi NQ, et al. Disruption of the cytochrome c gene in xylose-utilizing yeast Pichia stipitis leads to higher ethanol production. Yeast 15: 1021-1030, 1999. PubMed: 10455226

Jeffries TW, et al. Genome sequence of the lignocellulose-bioconvertingand xylose-fermenting yeast Pichia stipitis. Nat. Biotech. 25: 319-326, 2007. PubMed: 17334359

View All Curated Citations for this Product

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