Vibrio cholerae Pacini
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2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate an additional broth tube, a #3 agar slant and/or a plate.
5. Incubate all tubes and plate at 37°C for 24 hours.
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cholera patient, Madras, India
non O:1 [NAG]
Ramamurthy T, et al. Emergence of novel strain of Vibrio cholerae with epidemic potential in southern and eastern India. Lancet 341: 703-704, 1993. PubMed: 8095620