Azotobacter salinestris Page and Shivprasad
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2. Using a single tube of #1771 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #1771 agar slant and/or plate.
5. Incubate the tubes and plate at 26oC for 48-72 hours.
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Page WJ. Sodium-dependent growth of Azotobacter chroococcum. Appl. Environ. Microbiol. 51: 510-514, 1986.
Page W, Shivprasad S. Azotobacter salinestris sp. nov., a sodium-dependent, microaerophilic, and aeroadaptive nitrogen-fixing bacterium. Int. J. Syst. Bacteriol. 41: 369-376, 1991.
Page WJ, et al. Sodium-dependent Azotobacter chroococcum strains are aeroadaptive, microaerophilic, nitrogen-fixing bacteria. Appl. Environ. Microbiol. 54: 2123-2128, 1988.
Shivprasad S, Page WJ. Catechol formation and melanization by Na+-dependent Azotobacter chroococcum: a protective mechanism for aeroadaptation?. Appl. Environ. Microbiol. 55: 1811-1817, 1989.