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Chlorobaculum tepidum (Wahlund et al.) Imhoff


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Type strain. Genome sequenced strain.
Product category
Product type
Strain designation
TLS [CIP 105973]
Type strain
Genome sequenced strain
Isolation source
Travelodge Stream in Sulphur Bay
Geographical isolation
New Zealand; North Island
Product format
Storage conditions
-80°C or colder
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information


Specific applications


Extremophile type
Genome sequenced strain

Handling information

Handling procedure
  1. Open thawed vial according to enclosed instructions or visit for instructions.
  2. Remove 6 mL of pre-mixed #37 media from primary tube and aseptically transfer the entire contents of thawed vial to that tube. Additional test tubes can be inoculated by transferring up to 2 mL of the primary broth tube to secondary broth tubes. Fill the test tubes to capacity with additional #37 broth, leaving a small air bubble for expansion. Seal the test tube with a screw cap.
  3. Incubate at 45°C under a tungsten lamp for 3-7 days. The light should be on a 12 hour cycle (12 hours on/ 12 hours off). Incubate one #260 agar plate aerobically at 37°C to check for contamination.
  4. Use 10-20% of an actively growing culture to inoculate fresh tubes of medium.
Handling notes
ATCC Medium #37 may be stored separately as solution 1, solution 2/3 and solution 4. In a small screw cap tube, mix 5 mL of solution 1, 3 mL of solution 2/3, 0.2 mL of solution 4, and reduce the media with 0.1 mL of a 10% Thiosulphate solution. Place tubes in the dark for 24 hours prior to incoulation. A hazy, white precipitate should develop in the tubes overnight.

After 3-7 days, growth is evident by turbidity and a deep green pigmentation throughout the broth. When examined microscopically, the cells appear as small short rods in singles and pairs and are non-motile. The culture should be fed sodium sulfide at least once a week. This culture is oxygen-tolerant therefore strictly anoxic conditions are not required when using a large inoculum.

Additional information on this culture is available on the ATCC® web site at

Quality control specifications

Verification method
Whole-genome Sequencing


Deposited as
Chlorobium tepidum Wahlund et al.
MT Madigan
Type of isolate
Cross references
GenBank AF065617 Chlorobium tepidum dinitrogenase reductase (nifH) gene, partial cds.
GenBank AJ300491 Chlorobium tepidum partial pscB gene for putative iron-sulfur protein (PscB), strain ATCC 49652.
GenBank AF287481 Chlorobium tepidum phosphoenolpyruvate carboxylas (pepC) gene, partial cds; cytochrome C (pscC) and Orf117 genes, complete cds; and Orf73 gene, partial cds.
GenBank AF287480 Chlorobium tepidum glutamyl-tRNA synthetase (sye) gene, partial cds; and hydroxyneurosporene synthase (crtC), cytochrome b6f complex Rieske iron-sulfur protein subunit (petC), and cytochrome b6f complex cytochrome b subunit (petB) genes,
GenBank AF287482 Chlorobium tepidum Orf79 gene, partial cds; light-independent protochlorophyllide reductase N subunit (bchN), light-independent protochlorophyllide reductase B subunit (bchB), and light-independent protochlorophyllide reductase L subunit (bchL)
GenBank AE006470 Chlorobium tepidum TLS, complete genome.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.


Frequently Asked Questions


Curated Citations

Validation list no. 59. Int. J. Syst. Bacteriol. 46: 1189-1190, 1996.

Wahlund TM, et al. A thermophilic green sulfur bacterium from New Zealand hot springs, Chlorobium tepidum sp. nov.. Arch. Microbiol. 156: 81-90, 1991.

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