Flavobacterium columnare (Bernardet and Grimont 1989) Bernardet et al. 1996
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2. Using a single tube of #1750 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #1750 agar slant and/or plate.
5. Incubate the tubes and plate at 20°C for 72 hours. Broth tubes will do better if gently shaken.
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Bernardet JF. Flexibacter columnaris first description in France and comparison with bacterial strains from other origins. Dis. Aquat. Org. 6: 37-44, 1989.
Bernardet JF, Grimont PA. Deoxyribonucleic acid related ness and phenotypic characterization of Flexibacter columnaris sp. nov., nom. rev., Flexibacter psychrophilus sp. nov., nom. rev., and Flexibacter maritimus Wakabayashi, Hikida, and Masumura. Int. J. Syst. Bacteriol. 39: 346-354, 1989.