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Helicobacter pylori (Marshall et al.) Goodwin et al.


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Product category
Strain designation
T81213-NTB [CCUG 45423]
Type strain
Yes; type strain of Helicobacter nemestrinae
Genome sequenced strain
Isolation source
Gastric mucosa of pigtailed macaque, Macaca nemestrina
Enteric disease research
Infectious disease research
Product format
Storage conditions
2°C to 8°C
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information


Specific applications
Enteric Research


Helicobacter nemestrinae is a junior heterotypic synonym of Helicobacter pylori.

Handling information

Handling procedure
1. Open vial according to enclosed instructions or see for instructions. Aseptically rehydrate the vial contents using approximately 0.5 mL of #1705 broth.
2. Aseptically transfer the entire suspension into a tube of #1705 broth (5-6 mL). Mix well. This suspension can now be used to inoculate agar slant(s), plate(s), or the preferred biphasic culture. Two #1705 plates should be inoculated, one for microaerophilic growth and the second for aerobic growth. No growth should occur on the plate incubated aerobically.
3. To obtain a biphasic culture, add 0.6 mL of the suspension to a #1705 slant. The resulting pool at the
bottom of the slant is where the best, most rapid growth will occur.
4. Incubate at 37°C under microaerophilic conditions using an anaerobe jar with an active catalyst and a
microaerophilic gas generator pack, or other acceptable method, to obtain microaerophilic conditions.
Incubate tubes with caps loose.
5. Within 3 days, good growth should be obtained in the broth pool at the bottom of the slant. Additional
incubation may be required for colonies to appear on the agar plate. Further subcultures can be made
using the broth pool as the inoculum source. Subcultures to biphasic cultures will require only 24 to
48 hours of incubation for good growth.
Handling notes
The genus Campylobacter was described by Sebald and Veron in 1963 (Ann. Inst. Pasteur (Paris) 105: 897-910, 1963). Over the next twenty-five years, several species were assigned to that genus, among them C. fetus, C. coli, C. jejuni, C. sputorum, C. hyointestinalis, C. butzleri, C. lari, C. mucosalis and C. nitrofigilis. In the last few years, molecular examination of the strains has resulted in the transfer of some of these species into other genera, namely Helicobacter and Arcobacter (Int. J. Syst. Bacteriol. 41: 88-103, 1991 and ibid., 39: 397-405, 1989). Two species of Wolinella, W. recta and W. curva, have also been transferred into the genus Campylobacter (Ibid., 41: 88-103, 1991).
Additional information on this culture can be found on the ATCC® website at

Quality control specifications

Verification method
Whole-genome Sequencing


Deposited as
Helicobacter nemestrinae Bronsdon et al.
MA Bronsdon
Type of isolate
Cross references
GenBank X67854 H.nemestrinae (ATCC 49396T) gene for 16S rRNA.
GenBank AF348617 Helicobacter nemestrinae 16S ribosomal RNA gene, partial sequence.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.


Frequently Asked Questions


Curated Citations

Zhang Z, et al. Reclassification of Thermomonospora and Microtetraspora. Int. J. Syst. Bacteriol. 48: 411-422, 1998. PubMed: 9731279

Mendes EN, et al. Helicobacter trogontum sp. nov., isolated from the rat intestine. Int. J. Syst. Bacteriol. 46: 916-921, 1996. PubMed: 8863417

Bronsdon MA, et al. Helicobacter nemestrinae sp. nov., a spiral bacterium found in the stomach of a pigtailed macaque (Macaca nemestrina). Int. J. Syst. Bacteriol. 41: 148-153, 1991. PubMed: 1995031

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