Aeromonas salmonicida subsp. salmonicida (Lehmann and Neumann) Griffen et al.
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2. Using a single tube of #18 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate pellet.
3. Aseptically transfer this aliquot back into the broth tube and mix well. Transfer 0.5 ml to a second broth tube and mix well. Transfers to solid media can be made from the second tube.
4. Incubate broth tubes, slants and plates at 22oC for 48 hours.
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Chapman PF, et al. Isolation and phenotypic characterization of an oxidase-negative Aeromonas salmonicida causing furunculosis in coho salmon (Oncorhynchus kisutch). J. Wildl. Dis. 27: 61-67, 1991. PubMed: 1850808