Desulfotomaculum acetoxidans Widdel and Pfennig
ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Anaerobic conditions for transfer may be obtained by either of the following:
Loose screw caps on test tubes in anaerobic chamber,
Once growth has been detected, it has been noted that adding 0.1 mL of a 1.5 % Na2S.9H2O stock solution for each 10 mL of medium may enhance growth.
Always use freshly prepared anaerobic media.
For best results, use an anaerobe chamber. If one is not available, use a gassing cannula system. Using an anaerobic jar after rehydration of the freeze-dried pellet is not recommended. Once the culture has been established, using an anaerobic jar will work if the inoculum is 20% or greater.
A culture that has good growth and is fresh can be maintained at 4oC for up to a week. The cells can be stored at -70oC to -80oC by growing a large volume in #1964 broth, harvesting the cells, and then mixing the cell pellet in an equal volume of fresh #1964 and 20% glycerol (10% final glycerol concentration). Distribute the cells into vials (approximately 0.5 to 1.0 mL per vial), and freeze rapidly. Both the #1964 broth and glycerol need to be pre-reduced. This may be accomplished by adding 0.1 mL (for each 5 to 6 mL medium) of a 1.5% sodium sulfide solution.
Additional information on this culture is available on the ATCC® web site at www.atcc.org.
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Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.