Lactococcus garvieae (Collins et al.) Schleifer et al.
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2. Using a single tube of #44 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #260 agar slant and/or plate.
5. Incubate the tubes and plate at 37°C for 24 hours.
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Kusuda R, et al. Enterococcus seriolicida sp. nov., a fish pathogen. Int. J. Syst. Bacteriol. 41: 406-409, 1991. PubMed: 1883715
Bull. Jpn. Soc. Sci. Fish. 42: 1345-1352, 1976.
Eldar A, et al. Enterococcus seriolicida is a junior synonym of Lactococcus garvieae, a causative agent of septicemia and meningoencephalitis in fish. Curr. Microbiol. 32: 85-88, 1996. PubMed: 8574132
type strain of Enterococcus seriolicida