Thermotoga neapolitana Jannasch et al.
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Over pressurizing the culture by 1-2 atm. promotes growth.
Avoid using gases with hydrogen. Hydrogen has been reported to inhibit the growth of Thermotoga.
Growth can be detected with the increase of turbidity or microscopically at 1000X. After growth is achieved, remove the culture and place it at room temperature. If kept at high temperatures after growth, cell lysis will occur.
Additional information on this culture is available on the ATCC web site at www.atcc.org.
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Validation list no. 28. Int. J. Syst. Bacteriol. 39: 93-94, 1989.
Jannasch HW, et al. Thermotoga neapolitana New species of the extremely thermophilic eubacterial genus Thermotoga. Arch. Microbiol. 150: 103-104, 1988.
Belkin S, et al. A new sulfur-reducing, extremely thermophilic eubacterium from a submarine thermal vent. Appl. Environ. Microbiol. 51: 1180-1185, 1986.