Rhodospirillum centenum Favinger et al.
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2. Let the tube sit at room temperature for 30 minutes before inoculating it with the rehydrated culture.
3. Aseptically take 0.5 ml of the pre-reduced medium and rehydrate the entire pellet.
4. Transfer the rehydrated pellet back into the screw cap test tube and close tightly. (The test tube should be filled to capacity).
5. Incubate the culture at 30oC under a tungsten lamp.
When examined microscopically, the cells appear as spiral-shaped rods, in singles and pairs that are motile.
After four to seven days, growth is evident by turbidity and a deep red pigmentation throughout the broth. Once growth has been observed, the culture should be transferred to fresh broth. Subsequent growth should be detected within 48 to 72 hours.
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Validation list no. 48. Int. J. Syst. Bacteriol. 44: 182-183, 1994.
Favinger J, et al. Rhodospirillum centenum, sp. nov., a thermotolerant cyst-forming anoxygenic photosynthetic bacterium. Antonie van Leeuwenhoek 55: 291-296, 1989. PubMed: 2757370
Nickens D, et al. Biotype of the purple nonsulfur photosynthetic bacterium, Rhodospirillum centenum. Arch. Microbiol. 165: 91-96, 1996.
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