Legionella birminghamensis Wilkinson et al.
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Strain shows growth by diffuse turbidity in the supernatant portion of the broth.
Legionella species are nutritionally fastidious and are inhibited by media of poor caliber, so strict attention should be paid to the quality of the ingredients. The pH of the medium should be checked when cool, and is most critical. Unbuffered medium should not be used. Exposure of medium to light, particularly when hot, may also result in the accumulation of peroxides which can be inhibitory to the bacteria.
Before rehydration, vials may be stored at +2 to +8°C for extended periods with minimal loss of viability. Subculturing to maintain culture is discouraged because of the possibility of mutation, selection of variants, or contamination. To minimize changes, it is recommended that cells be harvested and stored at ‑70°C or below.
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Validation list no. 25. Int. J. Syst. Bacteriol. 38: 220-222, 1988.
Wilkinson HW, et al. Legionella birminghamensis sp. nov. isolated from a cardiac transplant recipient. J. Clin. Microbiol. 25: 2120-2122, 1987. PubMed: 3320081