• Quick Order
  • Careers
  • Support

Thermotoga maritima Huber et al.


Download Genome Learn about the ATCC Genome Portal
Type strain. Genome sequencing strain.
Product category
Product type
Strain designation
DSM 3109 [MSB8]
Type strain
Genome sequenced strain
Isolation source
Anaerobic marine mud
Geographical isolation
Italy; Vulcano Island
Product format
Storage conditions
-80°C or colder
Buy Now
Price: $620.00 EA
Discounts may be available for our fellow nonprofit organizations. Login to see your price.

Generally ships within 1-3 business days


ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information


Specific applications


Extremophile type
Sulfate reducer
Genome sequenced strain

Handling information

Handling procedure
  1. Sterilize the top of the Balch tube by spraying it with 70% ethanol and then flaming the top.
  2. If needed exchange the gas in the test tube for 100% N2.
  3. If the medium is pink (see discussion about resazurin) add 2.0 mL of reducing agent (3% cysteine, stock solution) per 100 mL of medium.  Let the medium sit at room temperature for 10 to 20 minutes - until the resazurin becomes colorless - before inoculating.
  4. When the Balch tube is ready to be inoculated let the frozen vial thaw at room temperature under a gentle stream of sterile oxygen-free gas.
  5. Using a 1.0 mL syringe tipped with a 22 gauge needle, withdraw the cell suspension from the vial, transfer it to the broth, and incubate at 70°C.  Plate 0.1 mL of the inoculated culture onto a non-selective medium and incubate aerobically at 37°C.
  6. Growth should be detected in the broth within 48 to 96 hours.  No growth should be detected on the aerobic plate or broth.



  1. Balch tube refers to a special type of test tube that is designed to be pressurized and is suited for anaerobic work.  Balch test tubes can be purchased form Bellco glass ( stock no. 2048-00150).
  2. Resazurin is a commonly used redox indicator which is pink when the redox potential is above –50 mv, and colorless when the redox potential is below –110 mv., i.e. highly reducing.  Most strict anaerobes require this low redox potential for optimal growth.
  3. To obtain a fully reduced medium, it is necessary that the medium be anoxic and that a reducing agent be added.  Common reducing agents are sodium sulfide, cysteine, dithiothreitol, and titanium citrate.
  4. Syringes can be made anaerobic by one of two methods.
    1. Displace the dead space in the syringe with a sterile oxygen-free gas.
    2. Displace the dead space in the syringe with a reducing agent.
Handling notes
Cells appear as single or paired rods surrounded with a sheath-like structure which protrudes balloon-like beyond the ends of the cells.

Mercaptoethane sulfonic acid, also known as Co-enzyme M, at a final concentration of 0.05% (Sigma M-1511) can also be used as a reducing agent.  It has been found to increase cell recovery.

Hydrogen is inhibitory to growth.

Purified genomic DNA of this strain is available as ATCC® 43589D-2™.
Additional information on this culture is available on the ATCC® web site at

Quality control specifications

Verification method
Whole-genome Sequencing


Deposited as
Thermotoga maritima Huber et al.
Chain of custody
ATCC <-- DSMZ <-- KO Stetter
Type of isolate
Year of origin
Cross references
GenBank AE000512 Thermotoga maritima MSB8, complete genome

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.


Frequently Asked Questions


Curated Citations

Validation list no. 22. Int. J. Syst. Bacteriol. 36: 573-576, 1986.

Nelson KE. Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima. Nature 399: 323-329, 1999. PubMed: 10360571

Huber R, et al. Thermotoga maritima sp. nov. represents a new genus of unique extremely thermophilic eubacteria growing up to 90 degrees C. Arch. Microbiol. 144: 324-333, 1986.

type strain

DSMZ, personal communication

Need assistance with this product? Contact our Technical Support team.


US and Puerto Rico

Outside the US

Hours of Operation

9:00am - 5:00pm
US Eastern Time