Campylobacter coli (Doyle) Veron and Chatelain
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
2. Using a single tube of #1115, #177, or #18 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #260 agar slant and/or plate.
5. Or, to obtain a biphasic culture, add 0.5 ml of the suspension to a #260 agar slant (see notes).
6. Incubate tubes and plate at 37oC, under microaerophilic conditions, for 24 to 48 hours. Use an anaerobe jar with an active catalyst and a microaerophilic gas generator pack, or other acceptable method. Incubate slant with cap loose.
Growth on agar takes longer than in biphasic culture. Once good growth is present, these organisms tend to lose viability, especially if exposed to air for lengthy periods. Colonies on #260 plate are circular, entire, small, opaque, and alpha hemolytic.
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