Campylobacter jejuni subsp. jejuni (Jones et al.) Veron and Chatelain
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2. Using a single tube of #1115, #177, or #18 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #260 agar slant and/or plate.
5. Or, to obtain a biphasic culture, add 0.5 ml of the suspension to a #260 agar slant (see notes )
6. Incubate tubes and plate at 37oC, under microaerophilic conditions, for 24 to 48 hours. Use an anaerobe jar with an active catalyst and a microaerophilic gas generator pack, or other acceptable method. Incubate slant with cap loose.
Growth on agar takes longer than with the biphasic culture. Once good growth is present, these organisms tend to lose viability, especially if exposed to air for lengthy periods.
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Penner JL, et al. Serotyping of Campylobacter jejuni and Campylobacter coli on the basis of thermostable antigens. Eur. J. Clin. Microbiol. 2: 378-383, 1983. PubMed: 6628376