Pectobacterium wasabiae (Goto and Matsumoto) Gardan et al.
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2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a second tube of broth, a slant, and/or plate.
5. Incubate all tubes and plate at 26°C for 24 hours.
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Goto M, Matsumoto K. Erwinia carotovora subsp. wasabiae subsp. nov. isolated from diseased rhizomes and fibrous roots of Japanese horseradish (Eutrema wasabi). Int. J. Syst. Bacteriol. 37: 130-135, 1987.
Validation list no. 68. Int. J. Syst. Bacteriol. 49: 1-3, 1999.
Hauben L, et al. Phylogenetic position of phytopathogens within the Enterobacteriaceae. Syst. Appl. Microbiol. 21: 384-397, 1998. PubMed: 9779605
Young JM, et al. Names of plant pathogenic bacteria 1864-1995. Rev. Plant Pathol. 75: 721-763, 1996.
Gardan L, et al. Elevation of three subspecies of Pectobacterium carotovorum to species level: Pectobacterium atrosepticum sp. nov., Pectobacterium betavasculorum sp. nov. and Pectobacterium wasabiae sp. nov.. Int. J. Syst. Evol. Microbiol. 53: 381-391, 2003. PubMed: 12710602