Streptococcus gallolyticus subsp. pasteurianus (Poyart et al.) Schlegel et al.
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2. From a single tube of #44 broth (5 to 6 ml), withdraw approximately 0.5 to 1 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #260 agar slant and/or plate.
5. Incubate all tubes and plate at 37°C for 24 hours.
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Knight RG, Shlaes DM. Physiological characteristics and deoxyribonucleic acid relatedness of human isolates of Streptococcus bovis and Streptococcus bovis (var.). Int. J. Syst. Bacteriol. 35: 357-361, 1985.
Schlegel L, et al. Reappraisal of the taxonomy of the Streptococcus bovis/Streptococcus equinus complex and related species: description of Streptococcus gallolyticus subsp. gallolyticus subsp. nov., S. gallolyticus subsp. macedonicus subsp. nov. and S. gallolyticus subsp. pasteurianus subsp. nov.. Int. J. Syst. Evol. Microbiol. 53: 631-645, 2003. PubMed: 12807180