Rhodococcus rhodochrous (Zopf) Tsukamura emend. Rainey et al.
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2. Using a single tube of #44 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a second tube of broth and a slant, and/or plate of #27 agar.
5. Incubate all tubes and plate at 37oC for 3 to 5 days.
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Badia DJ, Sardinas JL. Oxidation of steroid compounds in presence of antibiotics. US Patent 3,010,876 dated Nov 28 1961
Gordon RE, Mihm JM. A comparison of four species of mycobacteria. J. Gen. Microbiol. 21: 736-748, 1959. PubMed: 13851229
Goodfellow M, Alderson G. The actinomycetes-genus Rhodococcus: a home for the "rhodochrous" complex. J. Gen. Microbiol. 100: 99-122, 1977. PubMed: 874450
Heinzen RJ, Ensign JC. Effect of growth substrates on morphology of Nocardia corallina. Arch. Microbiol. 103: 209-217, 1975. PubMed: 1147739