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Teredinibacter turnerae Distel et al. 2002, sp. nov.

39867

Product category
Bacteria
Strain designation
T7901
Type strain
No
Isolation source
Shipworm, Bankia gouldi, from Newport River; Duke Marine Laboratory
Geographical isolation
United States; North Carolina; Beaufort
Product format
Frozen
Storage conditions
-80°C or colder
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
Degrades cellulose
Preceptrol
No

Characteristics

Comments
This culture was originally deposited as Strain T7902. Comparison of 10kb of contiguous sequence including the 3 end of the 23S rRNA, the 23S-5S spacer, the 5S rRNA and 12 subsequent contiguous open reading frames from ATCC 39867 with the genome sequence determined for Strain T7901 obtained from the original stock in the Waterbury collection revealed identical sequence alignement. However, ATCC 39867 differed by ~6% in nucleotide sequence from T7902 over 8kb of homologous protein coding sequence from the same genome region. Further, comparison of the ribotype patterns from ATCC 39867 and the original stocks of T7901 and T7902 from the Waterbury collection demonstrated that ATCC 39867 matched the T7901 pattern and differed from T7902. Personal communication Daniel L. Distel, Ph.D. Executive Director, Ocean Genome Legacy (April 2009)
Deposited as type strain
Technical information
ATCC Technical Services does not have technical information on patent deposits that are not produced or characterized by ATCC. Additional information can be found in the corresponding patent available from the patent holder or with the U.S. and/or international patent office.

Handling information

Medium
Temperature
30°C
Atmosphere
Aerobic
Handling procedure

  1. Open thawed vial according to enclosed instructions or visit www.atcc.org for instructions.
  2. Aseptically transfer the entire contents to a 5-6 mL tube of #1983 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary tubes.
  3. Use several drops of the primary broth tube to inoculate a #1983 plate and/or #1983 agar slant.
  4. Incubate at 30°C for 48-72 hours.

Handling notes

Deposited as type strain.

Colonies on #1983 are pinpoint and difficult to observe due to the color of the agar; microscopic evaluation is recommended.  The broth does not become turbid when growth occurs; microscopic observation is required.  Cells are highly motile and may be difficult to observe.

This culture was originally deposited as Strain T7902. Comparison of 10 kb of contiguous sequence including the 3’ end of the 23S rRNA, the 23S-5S spacer, the 5S rRNA and 12 subsequent contiguous open reading frames from ATCC® 39867™ with the genome sequence determined for Strain T7901 obtained from the original stock in the Waterbury collection revealed identical sequence alignment. However, ATCC® 39867™  differed by ~6% in nucleotide sequence from T7902 over 8kb of homologous protein coding sequence from the same genome region. Further, comparison of the ribotype patterns from ATCC® 39867™ and the original stocks of T7901 and T7902 from the Waterbury collection demonstrated that ATCC® 39867™  matched the T7901 pattern and differed from T7902. (Personal communication Daniel L. Distel, Ph.D. Executive Director, Ocean Genome Legacy, April 2009)

Additional information on this culture is available on the ATCC® web site at www.atcc.org.

History

Deposited as
Teredinobacter turnerae Distel et al. 2002, sp. nov.
Depositors
JB Waterbury
Patent depository
This material was deposited with the ATCC Patent Depository to fulfill U.S. or international patent requirements. This material may not have been produced or characterized by ATCC.  As an International Depository Authority (IDA) for patent deposits, ATCC is required to complete viability testing only at time of initial deposit of patent material. Patent deposits are made available on behalf of the Depositor when the pertinent U.S. or international patent is issued, but material may not be used to infringe the patent claims.
Patent number
4,861,721
Type of isolate
Animal
Cross references
GenBank EU604078 Teredinibacter turnerae strain T7901 16S small subunit ribosomal RNA gene, partial sequence

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Disclosures
This material is cited in a US and/or international patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Depositor of the party to which the material was furnished.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

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References

Curated Citations

Waterbury JB, et al. Bacteria for cellulose digestion. US Patent 4,861,721 dated Aug 29 1989

Distel DL, et al. Phylogenetic characterization and in situ localization of the bacterial symbiont of shipworms (Teredinidae: Bivalvia) by using 16S rRNA sequence analysis and oligodeoxynucleotide probe hybridization. Appl. Environ. Microbiol. 57: 2376-2382, 1991. PubMed: 1722662

Distel DL, et al. Teredinibacter turnerae gen. nov., sp. nov., a dinitrogen-fixing, cellulolytic, endosymbiotic gamma-proteobacterium isolated from the gills of wood-boring molluscs (Bivalvia: Teredinidae). Int. J. Syst. Evol. Microbiol. 52(Pt 6): 2261-2269, 2002. PubMed: 12508896

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