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Lacticaseibacillus casei (Orla-Jensen) Zheng et al.


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Lacticaseibacillus casei strain 03 was isolated from dairy products. This whole-genome sequenced bacterial type strain produces acetoin and diacetyl.
Product category
Product type
Preceptrol culture
Bacteria, Terrabacteria group, Bacillota, Bacilli, Lactobacillales, Lactobacillaceae, Lacticaseibacillus
Strain designation
03 [7, IAM 12473, Orland L-323, R.P. Tittsler 303]
Type strain
Genome sequenced strain
Former name
Lactobacillus casei (Orla-Jensen) Hansen and Lessel
Isolation source
Dairy products
Quality control
Agricultural research
Product format
Storage conditions
2°C to 8°C
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information


Specific applications
Produces acetoin (acetylmethylcarbinol)
Produces diacetyl
Quality control strain

Potential plant growth promoting strain; Biofertilizer



Alpha-acetolactate biosynthesis

Handling information

95% Air, 5% CO2
Handling procedure
  1. Open vial.
  2. Rehydrate the entire pellet with approximately 0.5 mL of #416 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #416 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary broth tubes.
  3. Use several drops of the primary broth tube to inoculate a #416 plate and/or #416 agar slant.
  4. Incubate at 37°C for 24 to 48 hours in an atmosphere of 5% CO2.
Handling notes
Additional information on this culture is available on the ATCC® web site at

Quality control specifications

Verification method
Whole-genome Sequencing


Deposited as
Lactobacillus casei subsp. casei (Orla-Jensen) Hansen and Lessel
GJ Hucker
Chain of custody
ATCC <-- GJ Hucker <-- S Orla-Jensen 7 (Streptobacterium casei)
Type of isolate
Food & Beverage
Cross references
GenBank Z80834 L.casei lacT gene.
GenBank AJ006018 Lactobacillus casei priA gene.
GenBank M23928 L.casei small subunit ribosomal RNA.
GenBank D16551 Lactobacillus casei DNA for 16S rRNA.
GenBank AJ003194 Lactobacillus casei ccpA & tnp genes.
GenBank Z75480 L.casei rrn operon, 23S-5S rRNA spacer (long).
GenBank Z75481 L.casei rrn operon, 23S-5S rRNA spacer (short).
GenBank Z75479 L.casei rrn operon, 16S-23S rRNA spacer (short).
GenBank AF129168 Lactobacillus casei sorbose operon, partial sequence.
GenBank M76708 L.casei lactate dehydrogenase (ldh) gene, complete cds.
GenBank Y18948 Lactobacillus casei hprK gene, lgt gene (partial) and 3 ORF's.
GenBank AF385770 Lactobacillus casei 16S ribosomal RNA gene, partial sequence.
GenBank Z75478 L.casei rrn operon, 16S-23S rRNA spacer (long), tRNA-Ile and tRNA-Ala genes.
GenBank U28137 Lactobacillus casei general catabolite repressor Ccpa protein (ccpA) gene, complete cds.
GenBank AJ251789 Lactobacillus casei bacteriophage A2 integration genes, lysis/lysogeny decision module and replication module genes.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

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Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.



Curated Citations

Hansen PA, Lessel EF. Lactobacillus casei (Orla-Jensen) comb. nov.. Int. J. Syst. Bacteriol. 21: 69-71, 1971.

Collins MD, et al. Deoxyribonucleic acid homology studies of Lactobacillus casei, Lactobacillus paracasei sp. nov., subsp. paracasei and subsp. tolerans, and lactobacillus rhamnosus sp. nov., comb. nov.. Int. J. Syst. Bacteriol. 39: 105-108, 1989.

Wayne LG. Actions of the Judicial Commission of the International Committee on Systematic Bacteriology on requests for opinions published between January 1985 and July 1993. Int. J. Syst. Bacteriol. 44: 177-178, 1994.

Alpert CA, Siebers U. The lac operon of Lactobacillus casei contains lacT, a gene coding for a protein of the Bg1G family of transcriptional antiterminators. J. Bacteriol. 179: 1555-1562, 1997. PubMed: 9045813

Auvray F, et al. Plasmid integration in a wide range of bacteria mediated by the integrase of Lactobacillus delbrueckii bacteriophage mv4. J. Bacteriol. 179: 1837-1845, 1997. PubMed: 9068626

View All Curated Citations for this Product

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