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YEp353

37725

Clone type
Vector
Applications
Molecular biology
Product format
Freeze-dried
Shipping information
Escherichia coli containing the plasmid
Storage conditions
2°C to 8°C
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Price: $550.00 EA
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
YE-type (episomal) shuttle vector
promoter-cloning vector
shuttle vector

Characteristics

Comments
The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): EcoRI-2; SmaI-3; BamHI-3; SalI-3; PstI-1; HindIII-2.
Restriction digests of the clone give the following sizes (kb): PstI--8.0; EcoRI--8.0; BamHI--8.0; HindIII--8.0.
One of 3 promoter-cloning, YE type shuttle vectors (ATCC 37725 - 37727) with URA3 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame.
The sequence and reading frame of the multiple cloning sequence is: 5' GA ATT CCC GGG GAT CCG TCG ACC TGC AGC CAA GCT TGC GAT CCC 3'. The sequence begins with the first nucleotide of the MCS and ends with the CCC for amino acid 8 of beta-galactosidase.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
7.943999767303467
Vector name
YEp353 (plasmid)
Type of vector
plasmid
Construction
pNM480, YEp352
Enhancer
none
Insert detection
lacZ
Markers
ampR; URA3
Promoters
none
Replicon
pMB1; 2 micron

Handling information

History

Depositors
CJ Lusty
Cross references
GenBank U03500

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

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Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Myers AM, et al. Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions. Gene 45: 299-310, 1986. PubMed: 3026915

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