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Acidocella facilis (Wichlacz et al.) Kishimoto et al.


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Product type
Strain designation
Type strain
Genome sequenced strain
Isolation source
Geographical isolation
United States; Pennsylvania
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information


Specific applications


Extremophile type
Mechanism of delta pH maintenance

Handling information

Handling procedure
1. Open vial according to enclosed instructions.                            

2. Using a single tube of #1421 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur pipette or 1.0 ml pipette.  Rehydrate the entire pellet. 

3. Aseptically transfer this aliquot back into the broth tube.  Mix well.

4. Incubate at 20°C for 5 to 6 days.

5. Once growth is achieved, additional transfers may be made.  Growth on agar will be sparse and slow.

Handling notes
Cells are actively motile rods with tapered ends.

          Growth is much better in broth than on solid medium.  Colonies are circular, white, entire, flat, and small with varying opacity.

Quality control specifications

Verification method
Whole-genome Sequencing


Deposited as
Acidiphilium facilis Wichlacz et al.
PL Wichlacz
Type of isolate
Cross references
GenBank M79369 Acidiphilium facilis 16S rRNA sequence.
GenBank M79370 Acidiphilium facilis 16S rRNA sequence.
GenBank M79371 Acidiphilium facilis 16S rRNA sequence.
GenBank AH001559 Acidiphilium facilis 16S rRNA sequence.
GenBank D30774 Acidiphilium facilis gene for 16S ribosomal RNA.
GenBank D16538 A. facilis recA gene for RecA protein, complete cds.

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Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


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If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.


Frequently Asked Questions


Curated Citations

Goulbourne E Jr., et al. Mechanism of delta pH maintenance in active and inactive cells of an obligately acidophilic bacterium. J. Bacteriol. 166: 59-65, 1986. PubMed: 3007441

Kishimoto N, et al. Transfer of Acidiphilium facilis and Acidiphilium aminolytica to the genus Acidocella gen.nov., and emendation of the genus Acidiphilium. Syst. Appl. Microbiol. 18: 85-91, 1995.

Shuttleworth KL, et al. Glucose catabolism in strains of acidophlic, heterotrophic bacteria. Appl. Environ. Microbiol. 50: 573-579, 1985.

Wichlacz PL, et al. Acidiphilium angustum sp. nov., Acidiphilium facilis sp. nov., and Acidiiphilium rubrum sp. nov.: Acidophilic heterotrophic bacteria isolated from acidic coal mine drainage. Int. J. Syst. Bacteriol. 36: 197-201, 1986.

Validation list no. 56. Int. J. Syst. Bacteriol. 46: 362-363, 1996.

View All Curated Citations for this Product

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