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Rhodovulum sulfidophilum (Hansen and Veldkamp) Hiraishi and Ueda


Product category
Product type
Strain designation
[DSM 1374; W4, LMG 5202]
Type strain
Isolation source
Marine mud flats
Geographical isolation
Product format
Mission Collection Item
This is a Mission Collection Item.


ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information


Specific applications
Produces hydrogen


Extremophile type
Sulfur oxidizer
Sulfate assimilation

Handling information

Under a tungsten lamp
Handling procedure
  1. Put 6 to 8 mL of ATCC Medium #1409 into a 13x100 mm screw cap test tube (small).  Add 0.1 mL cysteine (stock concentration 3%, 0.1 mL / 5-10 mL medium) and then fill the test tube to capacity with additional #1409.  Seal the test tube with a screw cap.
  2. Let the tube sit at room temperature for 30 minutes before inoculating it with the rehydrated culture.
  3. Open the freeze-dried vial according to enclosed instructions.
  4. Aseptically take 0.5 mL of the pre-reduced medium and rehydrate the entire pellet.
  5. Transfer the rehydrated pellet back into the screw cap test tube and close tightly.  (The test tube should be filled to capacity). 
  6. Incubate the culture at 26oC under a tungsten lamp (within 3 to 4 inches of the lamp).
  7. Once growth has been established (three to six days), the culture should be transferred to fresh broth.  If a large inoculum is used (10-20%) the medium does not need to be pre-reduced.
Handling notes
When examined microscopically, the cells are motile ovoids arranged singly, in pairs, and in chains. It may take up to two weeks to detect growth.

Growth on TSA and Sheep Blood agar may take up to two weeks. Colonies are variable in size, glistening, entire, circular, smooth, convex, and dark red in color.

Additional information on this culture is available on the ATCC® web site at


Deposited as
Rhodobacter sulfidophilus (Hansen and Veldkamp) Imhoff et al.
JF Imhoff
Chain of custody
ATCC <-- JF Imhoff <-- DSM 1374 <-- TH Hansen W4
Type of isolate

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.



Curated Citations

Hiraishi A, Ueda Y. Intrageneric structure of the genus Rhodobacter: transfer of Rhodobacter sulfidophilus and related marine species to the genus Rhodovulum gen. nov.. Int. J. Syst. Bacteriol. 44: 15-23, 1994.

Imhoff JF, et al. Rearrangement of the species and genera of the phototrophic "Purple nonsulfur bacteria". Int. J. Syst. Bacteriol. 34: 340-343, 1984.

Imhoff JF. Occurrence and evolutionary significance of 2 sulfate assimilation pathways in the Rhodospirillaceae. Arch. Microbiol. 132: 197-203, 1982.

Hansen TA, Veldkamp H. Rhodopseudomonas sulfidophila nov. spec., a new species of the purple nonsulfur bacteria. Arch. Mikrobiol. 92: 45-58, 1973. PubMed: 4725822

Stevens P, et al. Photoproduction of molecular hydrogen by Rhodobacter sulfidophilus. Syst. Appl. Microbiol. 8: 19-23, 1986.

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