• Quick Order
  • Resources
  • Support

Desulfobulbus propionicus Widdel


Product category
Product type
Strain designation
DSM 2032 [1 pr 3]
Type strain
Isolation source
Freshwater mud
Geographical isolation
Germany; West Germany
Product format
Storage conditions
2°C to 8°C
Mission Collection Item
This is a Mission Collection Item.


ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information


Specific applications


Extremophile type
Sulfate reducer

Handling information

80% N2, 20% CO2
Handling procedure
  1. Sterilize the top of the Balch tube (see below) by spraying it with 70% ethanol and then flaming the top.
  2. Exchange the gas in the test tube for the appropriate gas mixture.
  3. Add 0.1 to 0.2 mL of reducing agent (Cysteine 3% w./vl) per 10 mL of medium.  Let the medium sit at room temperature overnight before inoculating.
  4. When the Balch tube is ready to inoculate, open the vial according to enclosed instructions. 
  5. Perform all steps under anaerobic conditions.
  6. Aseptically transfer 0.5 mL of #2821 broth to the vial and rehydrate the pellet.  Transfer the suspension back into the broth tube.  Inoculate a plate of a non-selective medium such as Tryptic Soy, Nutrient, or blood agar with 0.1 mL of the cell suspension.
  7. Seal the tube with a rubber stopper and incubate anaerobically at 37°C.  Incubate the plate(s) aerobically as a purity check. No growth should occur on the aerobic plate.
  8. After two or three days, growth should be evident as indicated by faint turbidity throughout the broth. Once growth has been established the culture should be transferred to fresh broth every 72 to 96 hours.
  9. This culture is very sensitive to oxygen when initially rehydrated; therefore steps should be taken to avoid exposure to oxygen.  When the culture exhibits good growth it will remain viable for up to 1 week if stored at 4°C under anaerobic condition.


  1. Balch tube refers to a special type of test tube that is designed to be pressurized and is suited for anaerobic work.  The Balch test tubes can be purchased form Bellco galss (; stock no. 2048-00150).
  2. Resazurin is a commonly used redox indicator that is pink when the redox potential is above –50 mv., and colorless when the redox potential is below –110 mv. i.e. highly reducing.  Most strict anaerobes require this low redox potential for optimum growth.
  3. To obtain a fully reduced medium, it is necessary that the medium be anoxic and that a reducing agent be added.  Common reducing agents are sodium sulfide, cysteine, dithiothreitol, and titanium citrate.
  4. Syringes can be made anaerobic by one of two methods. 
    1. Displace the dead space in the syringe with a sterile oxygen-free gas.  
    2. Displace the dead space in the syringe with a reducing agent. 
  5. 100% nitrogen or 80% nitrogen-10% carbon dioxide-10%  hydrogen gas mixture is typically employed as the oxygen free gas source.
Handling notes
Cells are short rods with pointed ends.
Additional information on this culture is available on the ATCC web site at


Deposited as
Desulfobulbus propionicus Widdel
Chain of custody
ATCC <-- DSM <-- F Widdel strain Lindhorst
Type of isolate
Cross references
GenBank M34410 D.propionicus 16S ribosomal rRNA.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.


Frequently Asked Questions


Curated Citations

Validation list no. 7. Int. J. Syst. Bacteriol. 31: 382-383, 1981.

Moore WE, et al. Index of the bacterial and yeast nomenclatural changes published in the International Journal of Systematic Bacteriology since the 1980 approved lists of bacterial names (1 January 1980 to 1 January 1985). Int. J. Syst. Bacteriol. 35: 382-407, 1985.

Need assistance with this product? Contact our Technical Support team.



US and Puerto Rico

Outside the US

Hours of Operation

9:30am - 5:30pm
US Eastern Time