Legionella wadsworthii Edelstein et al.
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2. Using a single tube of #1099 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #1099 agar slant and/or plate.
5. Incubate the tubes and plate at 37°C in 5% CO2 for 1-3 days.
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Validation list no. 11. Int. J. Syst. Bacteriol. 33: 672-674, 1983.
Edelstein PH, et al. Legionella wadsworthii species nova: a cause of human pneumonia. Ann. Intern. Med. 97: 809-813, 1982. PubMed: 6756238