ATCC ATCC Logo 0
  • Quick Order

Mycoplasma genitalium Tully et al.

33530

Bacterial type strain with the ability to produce a surface attachment protein.
Product category
Bacteria
Strain designation
G37
Type strain
Yes
Genome sequenced strain
Yes
Isolation source
Urethra of male with non-gonococcal urethritis
Applications
Infectious disease research
Sexually transmitted disease research
Product format
Frozen
Storage conditions
-80°C or colder
Buy Now
Price: $414.00 EA
Discounts may be available for our fellow nonprofit organizations.

Generally ships within 1-3 business days

Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
Produces a surface attachment protein
Preceptrol
No

Characteristics

Comments
Genome sequenced strain

Handling information

Medium
Temperature
37°C
Atmosphere
Aerobic
Handling procedure
  1. We recommend that you use T-flasks in the propagation of Mycoplasma genitalium so that a larger surface area will be available for the cells to adhere to the glass or plastic surface. Approximately 10 mL of Medium #988 is placed in 25 cm2 flasks, and 200 mL can be placed in 125 cm2 flasks if larger amounts are desired.
  2. Add an additional 5% heat-inactivated fetal bovine serum to medium #988. Open the vial according to enclosed instructions.
  3. Transfer the contents of the vial to a 25 cm2 flask. 
  4.  Incubate the flask in a horizontal position at 37°C for 7-14 days. Note: This strain can be slow to establish growth, however subsequent transfers should grow faster once primary growth is established.  
  5. After the incubation period,  observe the flask for flocs in the medium and for cells adhering to the bottom of the flask.  Growth is also indicated by an indicator change from red to yellow.  
  6. To prepare the culture for subculturing and/or scaling-up to larger volumes, loosen adherent cells with a suitable, sterile scraper.  Centrifuge the culture and remove the supernatant (spent growth media).  Resuspend the pellet with fresh medium and place  into a new 25 cm2 flask with the appropriate volume of fresh medium.  
  7. Incubate the flask in a horizontal position at 37°C for 7-14 days. 
  8. When the medium has turned yellow, loosen adherent cells with a suitable, sterile scraper.  Use a 10% inoculum when subculturing this item and/or scaling-up to larger volumes to ensure healthy growth in subcultures.  
     
Handling notes
This strain requires an additional 5% heat-inactivated fetal bovine serum be added to ATCC Medium #988 to sustain growth of this strain. Growth is best acheived in T-25 flasks with 10 mL of medium and a 10% inoculum.
The depositor has suggested that the initial slow, poor adherent growth may be due to the cryoprotectant used in the freeze-drying process. Subsequent transfers show improvement in growth times.
This strain may need to have growth established first before sub-culturing.
Purified genomic DNA of this strain is available as ATCC® 33530D™.
Additional information on this culture is available on the ATCC® web site at www.atcc.org.

History

Deposited as
Mycoplasma genitalium Tully et al.
Depositors
JG Tully
Type of isolate
Human
Patient gender
Male
Cross references
GenBank L43967 Mycoplasma genitalium G37, complete genome.
GenBank M32341 M.genitalium Trp-tRNA-UGA gene.
GenBank X16463 Mycoplasma gentalium tuf gene for elongation factor tu.
GenBank M31431 M.genitalium attachment protein (MgPa) gene, complete cds.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Hu PC, et al. A Mycoplasma genitalium protein resembling the Mycoplasma pneumoniae attachment protein. Infect. Immun. 55: 1126-1131, 1987. PubMed: 2437033

Tully JG. Mycoplasma genitalium, a new species from the human urogenital tract. Int J Syst Bacteriol 33: 387-396, 1983.

Fraser CM, et al. The minimal gene complement of Mycoplasma genitalium. Science 270: 397-403, 1995. PubMed: 7569993

Frequently Asked Questions

Need assistance with this product? Contact our Technical Support team.

Telephone

Telephone

US and Puerto Rico

800-638-6597

Outside the US

+1-703-365-2700
hours

Hours of Operation

Monday-Friday
9:30am - 5:30pm
US Eastern Time