Campylobacter ureolyticus Vandamme et al.

1. Open vial.
2. Under anaerobic conditions, withdraw 0.5 mL of recommended broth from a single test tube (5 to 6 mL) and rehydrate the entire vial contents.
3. Aseptically transfer this aliquot back into the broth tube. A slant and a pre-reduced blood plate may also be inoculated with 0.1 mL each of the cell suspension. An aerobic blood plate may also be streaked to check for purity.
4. Incubate tubes and plate under anaerobic conditions at 37°C. Incubate a second #260 plate aerobically at 37°C.
5. Within two to three days, growth is should be evident by slight turbidity in the broth. Translucent, irregular, peaked and glistening colonies are formed on the anaerobic #260 plate. No growth should occur on the agar plate incubated aerobically.

ANAEROBIC CONDITIONS:

Anaerobic conditions for transfer may be obtained by either of the following:

• Use of an anaerobic gas chamber, or
• Placement of test tubes under a gassing cannula system hooked to anaerobic gas.

Anaerobic conditions for incubation may be obtained by any of the following:

• Loose screw caps on test tubes in anaerobic chamber,
• Loose screw caps on test tubes in an activated anaerobic gas pack jar, or
• Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.

Anaerobe Systems Brucella blood agar may be used for growth on agar.

According to Vandamme et al., Bacteroides ureolyticus Jackson and Goodman 1978 (Approved Lists 1980) is clearly a member of the family Campylobacteraceae Vandamme and De Ley 1991. However, the authors do not believe that it should be renamed at this time and prefer to consider this taxon a species incertae sedis.

Always use freshly prepared pre-reduced media or pre-reduced media that has been previously prepared but stored under anaerobic conditions. Resazurin in the media is a color indicator for anaerobic conditions. Observance of pink color in medium before use or during incubation shows anaerobic conditions have not been met and oxidation has occurred. Medium should be discarded.

Additional information on this culture is available on the ATCC^® web site at www.atcc.org.

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