• Quick Order
  • Careers
  • Support

Porphyromonas gingivalis (Coykendall et al.) Shah and Collins


Download Genome Learn about the ATCC Genome Portal
Porphyromonas gingivalis strain 2561 is a whole-genome sequenced bacterial type strain that was isolated from a human gingival sulcus. This culture is part of a set of quality control organisms for BioMerieux Vitek products.
Product category
Product type
Strain designation
Type strain
Genome sequenced strain
Isolation source
Gingival sulcus
Quality control
Product format
Storage conditions
-80°C or colder
Buy Now
Price: $288.00 EA
Discounts may be available for our fellow nonprofit organizations. Login to see your price.

Generally ships within 1-3 business days


ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information


Specific applications
Quality control strain


Invasion of gingival epithelial cells in culture

Genome sequenced strain

Component of ATCC MSA-1002, ATCC MSA-1003, and ATCC MSA-2002.

Handling information

Handling procedure
  1. Open thawed vial.
  2. Under anaerobic conditions aseptically transfer the entire contents to a 5-6 mL tube of #2722 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary broth tubes. Best practice dictates the use of pre-reduced media.
  3. Use several drops of the primary broth tube to inoculate a #260 plate and/or #260 agar slant.
  4. Incubate in an anaerobic atmosphere at 37°C for 2-5 days. Incubate one agar plate aerobically at 37°C to check for contamination.


Anaerobic conditions for transfer may be obtained by the use of an anaerobic gas chamber or placement of test tubes under a gassing cannula system connected to anaerobic gas.

Anaerobic conditions for incubation may be obtained by any of the following:

  • Loose screw caps on test tubes in an anaerobic chamber
  • Loose screw caps on test tubes in an activated anaerobic gas pack jar
  • Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained
Handling notes
Viability on agar may be hard to visualize with the naked eye. This organism produces punctiform colonies.
Anaerobe Systems Brucella Blood plate (AS-111 or AS-141) can be use to analyze colony morphology and purity.
No growth occurs on sheep blood agar aerobically. A black pigment can be observed on Brucella Blood agar or Laked Blood agar after 5 days incubation.
Additional information on this culture is available on the ATCC® web site at

Quality control specifications

Verification method
Whole-genome Sequencing


Deposited as
Bacteroides gingivalis Coykendall et al.
AL Coykendall
Chain of custody
ATCC <-- AL Coykendall <-- J Slots
Type of isolate
Cross references
GenBank AB016085 Porphyromonas gingivalis PorT, upp, and prtQ genes, complete cds.
GenBank AB035471 Porphyromonas gingivalis gene for EF-G, complete cds, strain:ATCC 33277.
GenBank AB035465 Porphyromonas gingivalis gene for EF-Tu, complete cds, strain:ATCC 33277.
GenBank L16492 Porphyromonas gingivalis ATCC 33277 16S ribosomal RNA gene, complete sequence.
GenBank X85186 P.gingivalis cpgR gene.
GenBank AB025779 Porphyromonas gingivalis gene for Dps, complete cds.
GenBank D90152 B. gingivalis superoxide dismutase (EC gene.
GenBank AB015879 Porphyromonas gingivalis dnaK operon genes, complete cds.
GenBank D17795 Porphyromonas gingivalis (strain ATCC 33277) fimA gene for fimbrilin, complete cds.
GenBank AB035476 Porphyromonas gingivalis gene for formate channel, complete cds, strain:ATCC 33277.
GenBank AB016284 Porphyromonas gingivalis mfa1 gene for 67kDa fimbrillin, complete cds, strain:ATCC 33277.
GenBank AF118635 Porphyromonas gingivalis isolate ATCC 33277 16S-23S intergenic spacer region, complete sequence.
GenBank AB016286 Porphyromonas gingivalis gene for 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase, complete cds.
GenBank AP009380 Porphyromonas gingivalis ATCC 33277 DNA, complete genome.
GenBank AB035459 Porphyromonas gingivalis gene for 16S rRNA, partial sequence, strain: ATCC 33277

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.


Frequently Asked Questions


Curated Citations

Tompkins GR, et al. Detection and comparison of specific hemin binding by Porphyromonas gingivalis and Prevotella intermedia. J. Bacteriol. 179: 620-626, 1997. PubMed: 9006012

Barkocy-Gallagher GA, et al. Analysis of the prtP gene encoding porphypain, a cysteine proteinase of Porphyromonas gingivalis. J. Bacteriol. 178: 2734-2741, 1996. PubMed: 8631659

Tran SD, Rudney JD. Multiplex PCR using conserved and species-specific 16S rRNA gene primers for simultaneous detection of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis. J Clin Microbiol 34: 2674-2678, 1996. PubMed: 8897163

Lamont RJ, et al. Porphyromonas gingivalis invasion of gingival epithelial cells. Infect. Immun. 63: 3878-3885, 1995. PubMed: 7558295

Shah HN, Collins MD. Proposal for reclassification of Bacteroides asaccharolyticus, Bacteroides gingivalis, and Bacteroides endodontalis in a new genus, Porphyromonas. Int. J. Syst. Bacteriol. 38: 128-131, 1988.

View All Curated Citations for this Product

Need assistance with this product? Contact our Technical Support team.


US and Puerto Rico

Outside the US

Hours of Operation

9:00am - 5:00pm
US Eastern Time