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Thermoanaerobacter acetoethylicus (Ben-Bassat and Zeikus) Rainey and Stackebrandt

33265

Thermoanaerobacter acetoethylicus strain HTB2 is a thermophilic type strain isolated in 1978 from a microbial mat at Yellowstone National Park. This strain produces methane.

Product category
Bacteria
Product type
Anaerobe
Extremophile
Strain designation
HTB2 [DSM 2359, HTB2/W]
Type strain
Yes
Isolation source
Microbial mat at Yellowstone National Park
Geographical isolation
United States; Wyoming
Product format
Freeze-dried
Storage conditions
2°C to 8°C
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Documentation

Biosafety Icon Biosafety Level 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
Produces methane
Preceptrol
No

Characteristics

Extremophile type
Thermophile

Handling information

Medium
Temperature
60°C
Atmosphere
Anaerobic
Handling procedure
  1. Open vial according to enclosed instructions or visit www.atcc.org for instructions.
  2. Under anaerobic conditions aseptically rehydrate the entire pellet with approximately 0.5 mL of #1135 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #1135 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary broth tubes. Best practice dictates the use of pre-reduced media.
  3. Use several drops of the primary broth tube to inoculate a #1135 plate and/or #1135 agar slant.
  4. Incubate in an anaerobic atmosphere at 60°C for 48 hours. Incubate one agar plate aerobically at 37°C to check for contamination.

ANAEROBIC CONDITIONS:
Anaerobic conditions for transfer may be obtained by the use of an anaerobic gas chamber or placement of test tubes under a gassing cannula system connected to anaerobic gas.

Anaerobic conditions for incubation may be obtained by any of the following:

  • Loose screw caps on test tubes in an anaerobic chamber
  • Loose screw caps on test tubes in an activated anaerobic gas pack jar
  • Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained

History

Deposited as
Thermobacteroides acetoethylicus Ben-Bassat and Zeikus
Depositors
JG Zeikus
Chain of custody
ATCC <-- JG Zeikus <-- P.W. Hegge
Type of isolate
Environmental
Year of origin
1978
Special collection
National Park Service (NPS) Special Collection
Cross references
GenBank X69336 T.acetoethylicus 16S rRNA.
GenBank L09163 Thermobacteroides acetoethylicus (ATCC 33265) 16S ribosomal RNA (16S rRNA) gene.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

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Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Material Transfer Agreement Addendum for National Park Service Products

For every order of this item, you must provide a signed Material Transfer Agreement Addendum for National Park Service Products. We cannot ship this item until we receive this addendum. The person signing the addendum as the principal investigator must match the end user as listed on the applicable sales order for the item. Email the signed addendum to [email protected] with a reference to both your account and sales order numbers. Once received, your addendum will be reviewed, and this item will be released for shipment if all requirements are met. If you need assistance with your order, please contact our Customer Care team or your applicable distributor.

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Validation list no. 11. Int. J. Syst. Bacteriol. 33: 672-674, 1983.

Ben Bassat A, Zeikus JG. Thermobacteroides acetoethylicus, new genus new species of chemoorganotrophic, anaerobic, thermophilic bacterium. Arch. Microbiol. 128: 365-370, 1981.

Rainey FA, Stackebrandt E. Transfer of the type species of the genus Thermobacteroides to the genus Thermoanaerobacter as Thermoanaerobacter acetoethylicus (Ben-Bassat and Zeikus 1981) comb. nov., description of Coprothermobacter gen. nov., and reclassification of Thermobacteroides proteolyticus as Coprothermobacter proteolyticus (Ollivier et al. 1985) comb. nov.. Int. J. Syst. Bacteriol. 43: 857-859, 1993.

Zeikus JG, et al. Microbiology of methanogenesis in thermal, volcanic environments. J. Bacteriol. 143: 432-440, 1980. PubMed: 7400098

algal-bacterial mat, Octopus Spring, Yellowstone National Park

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