Serpens flexibilis Hespell
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2. From a single tube of #972 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a second tube of broth, a slant and/or plate.
5. Incubate all tubes and plate at 30°C for 24 to 48 hours.
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Ahmad S, Jensen RA. Evolution of the biochemical pathway for aromatic amino acid biosynthesis in Serpens flexibilis in relationship to its phylogenetic position. Arch. Microbiol. 147: 8-12, 1987.
Hespell RB. Serpens flexibilis gen. nov., sp. nov., an unusually flexible, lactate-oxidizing bacterium. Int. J. Syst. Bacteriol. 27: 371-381, 1977.
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.