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Desulfovibrio vulgaris subsp. vulgaris Postgate and Campbell

29579

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Desulfovibrio vulgaris subsp. vulgaris was isolated from Wealden clay in England. This whole-genome sequenced bacterial type strain can be used in biotechnology. 
Product category
Bacteria
Product type
Anaerobe
Extremophile
Strain designation
NCIB 8303 [DSM 644, Hildenborough]
Type strain
Yes
Genome sequenced strain
Yes
Isolation source
Wealden clay
Geographical isolation
England
Applications
Bioinformatics
Product format
Freeze-dried
Storage conditions
2°C to 8°C
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
Biotechnology
Preceptrol
No

Characteristics

Extremophile type
Sulfate reducer
Comments
Genome sequenced strain
Bacteriophage induction
Reduction of tetravalent uranium

Handling information

Medium
Temperature
30°C
Atmosphere
Anaerobic
Handling procedure
  1. If the medium is not freshly prepared (less that 1 week), then it is a good idea to replace the head space with a fresh anaerobic gas such as 100% N2. A reducing agent should then be added to insure that anaerobic conditions are obtained.  It is suggested that a 3% stock solution of cysteine be added, 0.1 mL. for each 5-10 mL, to each tube(s) of medium.  Once that reducing agent has been added, let the tube(s) sit for a minimum of 1 hour before inoculation.
  2. Open vial according to enclosed instructions.
  3. Perform all steps under anaerobic conditions. (see below)
  4. Aseptically transfer 0.5 mL of Medium #1249 to the vial and rehydrate the entire freeze-dried pellet.  Transfer the suspension back into the tube of broth.  Inoculate a plate of non-selective medium with 0.1 of the culture.
  5. Seal the test tube with a rubber stopper and incubate anaerobically at 30°C.  Incubate the plate(s) aerobically as a purity check.
  6. After two or three days, growth should be evident as indicated by turbidity through out the broth.  Once growth has been established, the culture should be transferred to fresh broth every 24 to 48 hours.
  7. This culture is very sensitive to oxygen. Therefore, steps should be taken to avoid exposure to oxygen.  When the culture exhibits good growth, it will remain viable for up to 1 week if stored at 4°C under anaerobic conditions.

 

ANAEROBIC CONDITIONS:   

Anaerobic conditions for transfer may be obtained by either of the following:

  • Use of an anaerobic gas chamber.
  • Placement of test tubes under a gassing cannula system hooked to anaerobic gas.

Anaerobic conditions for incubation may be obtained by any of the following:

  • Loose screw caps on test tubes in anaerobic chamber.
  • Loose screw caps on test tubes in an activated anaerobic gas pack jar.
  • Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.
Handling notes

Use of a shaking incubator improves growth.

Anaerobe Systems PRAS Brucella Blood Plates (AS-111 or AS-141) can be used to analyze colony morphology and purity. 

A black precipitate may be observed because of the breakdown of ferrous ammonium sulfate. Ferrous ammonium sulfate is not required for this organism to grow.

Always use freshly prepared pre-reduced media or pre-reduced media that has been previously prepared but stored under anaerobic conditions. Resazurin in the media is a color indicator for anaerobic conditions. Observance of pink color in medium before use or during incubation shows anaerobic conditions have not been met and oxidation has occurred. Medium should be discarded.

Purified genomic DNA of this strain is available as ATCC 29579D-5.

Additional information on this culture is available on the ATCC® web site at www.atcc.org.

Quality control specifications

Verification method
Whole-genome Sequencing

History

Deposited as
Desulfovibrio vulgaris subsp. vulgaris Postgate and Campbell
Depositors
NCIMB
Chain of custody
ATCC <-- NCIMB <-- ME Adams strain Hildenborough
Cross references
GenBank AE017285 Desulfovibrio vulgaris subsp. vulgaris str. Hildenborough, complete genome.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Lovley DR, et al. Reduction of uranium by cytochrome c3 of Desulfovibrio vulgaris. Appl. Environ. Microbiol. 59: 3572-3576, 1993. PubMed: 8285665

Seyedirashti S, et al. Induction and partial purification of bacteriophages from Desulfovibrio vulgaris (Hildenborough) and Desulfovibrio desulfuricans ATCC 13541. J. Gen. Microbiol. 137: 1545-1549, 1991. PubMed: 1683398

Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.

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