Thermus thermophilus (Oshima and Imahori) Williams et al.


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Type strain. Used in enzyme studies for phosphoglucomutase, phosphofructokinase, and fructose 1,6-diphosphatase. Resistant to heavy metal ions. Produces thermostable D-xylose isomerase.and restriction endonuclease TthHB8I. Genome sequenced strain.
Product category
Product type
Strain designation
Type strain
Genome sequenced strain
Isolation source
Hot spring
Geographical isolation
Product format
Storage conditions
2°C to 8°C
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information


Specific applications
Produces D-xylose isomerase
Produces fructose-1,6-bisphosphatase
Produces phosphofructokinase
Produces phosphoglucomutase
Produces restriction endonuclease TtHB8I
Produces thermostable D-xylose isomerase


Extremophile type
Heat stability of tRNA and ribosomes
Respiratory proteins
Iron-sulfur protein
Resistance to heavy metal ions
Genome sequenced strain

Handling information

Handling procedure

  1. Open vial according to enclosed instructions or visit for instructions.
  2. Rehydrate the entire pellet with approximately 0.5 mL of #697 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #697 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary tubes.
  3. Use several drops of the primary broth tube to inoculate a #697 plate and/or #697 agar slant.
  4. Incubate at 70°C for 24-48 hours.

Handling notes
Bacteriophage may be present in culture. Plaques may be visible on 70°C agar plates.
Parafilm or tape agar cultures, plates and kolles, to prevent the agar from drying out.
Additional information on this culture is available on the ATCC® web site at

Quality control specifications

Verification method
Whole-genome Sequencing


Deposited as
Thermus thermophilus Oshima and Imahori
T Oshima
Chain of custody
ATCC <-- T Oshima
Type of isolate
Cross references
GenBank M35674 T.thermophilus 23S ribosomal RNA gene.
GenBank X58342 T.thermophilus 16S ribosomal RNA, part.
GenBank M33159 T.thermophilus insertion sequences Is1000A and Is1000B.
GenBank X06657 Thermus thermophilus HB8 tuf gene for elongation factor Tu (EF-Tu).
GenBank U17352 Thermus aquaticus L-glutamine:D-fructose-6-P amidotransferase (glmS) gene, complete cds and s-layer P100 (slp) gene, partial cds.
GenBank AP008226 Thermus thermophilus HB8 genomic DNA, complete genome.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


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Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.



Curated Citations

Chiong M, et al. Resistance of Thermus spp. to potassium tellurite. Appl. Environ. Microbiol. 54: 610-612, 1988.

Lehmacher A, Bisswanger H. Isolation and characterization of an extremely thermostable D-xylose isomerase from Thermus aquaticus HB 8. J. Gen. Microbiol. 136: 679-686, 1990.

Yoshida M, et al. The thermostable allosteric enzyme: phosphofructokinase from an extreme thermophile. Biochem. Biophys. Res. Commun. 43: 36-39, 1971. PubMed: 4252961

Yoshida M, Oshima T. The thermostable allosteric nature of fructose 1,6-diphosphatase from an extreme thermophile. Biochem. Biophys. Res. Commun. 45: 495-500, 1971. PubMed: 4334342

Sato S, et al. A DNA methylase from Thermus thermophilus HB8. J. Biochem. 88: 737-747, 1980. PubMed: 6448253

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