Pirellula staleyi Schlesner and Hirsch
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2. Using a single tube of #36 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Transfer 1.0 ml of the suspension to a second tube of broth. From the second tube, use several drops to inoculate a slant and/or plate if desired.
5. Incubate the broth at 26oC for 7 days. Growth on agar may take up to 2 weeks.
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Schlesner H, Hirsch P. Rejection of the genus name Pirella for pear-shaped budding bacteria and proposal to create the genus Pirellula gen. nov.. Int. J. Syst. Bacteriol. 37: 441, 1987.
Liesack W, et al. Chemical composition of the peptidoglycan-free cell envelopes of budding bacteria of the Pirella and Planctomyces group. Arch. Microbiol. 145: 361-366, 1986.
Starr MP, et al. Assignment of ATCC 27377 to Planctomyces staleyi sp. nov. and conservation of Pasteuria ramosa Metchnikoff 1888 on the basis of type descriptive material. Request for an Opinion. Int. J. Syst. Bacteriol. 33: 666-671, 1983.
Schlesner H, Hirsch P. Assignment of ATCC 27377 to Pirella gen. nov. as Pirella staleyi comb. nov.. Int. J. Syst. Bacteriol. 34: 492-495, 1984.
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.