Lactobacillus paracasei subsp. paracasei Collins et al.
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2. From a single tube of #416 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the entire pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well
4. Use several drops of this suspension to inoculate a second tube of broth, a slant and/or a plate.
5. Incubate tubes and plate at 37oC in an atmosphere of 5% CO2 for 24 to 48 hours. Loosen screw caps of all test tubes during the incubation period. The CO2 atmosphere is not necessary for growth, but may be helpful.
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Mills CK, Lessel EF. Designation and description of the type strain of Lactobacilllus casei subsp. alactosus Rogosa et al.. Int. J. Syst. Bacteriol. 23: 67-68, 1973.
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.
type strain of Lactobacillus casei subsp. alactosus