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Staphylococcus aureus subsp. aureus Rosenbach


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Staphylococcus aureus subsp. aureus strain Seattle 1945 is a whole-genome sequenced clinical isolate.  This culture is used as a quality control strain for the CAMP test, assay of wood smoke concentrate, evaluation of Mueller-Hinton agar, examination of dairy products, media testing, CLSI disk diffusion, and for Abbott, and Autobac products.
Product category
Product type
Preceptrol culture
Strain designation
Seattle 1945
Type strain
Genome sequenced strain
Isolation source
Clinical isolate
Enteric disease research
Infectious disease research
Media testing
Quality control
Water testing
Product format
Storage conditions
2°C to 8°C
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information


Specific applications
CAMP test
Assay of wood smoke concentrate
Control strain
Control strain for identification
Evaluation of Mueller-Hinton agar
Examination of dairy products
Media testing
Quality control strain
Reference material
Susceptibility disc testing
Enteric Research
Water testing
Susceptibility disc testing ampicillin
Susceptibility disc testing caryomycin
Susceptibility disc testing cephalexin
Susceptibility disc testing cephaloglycin
Susceptibility disc testing cephaloridine cephalomycin
Susceptibility disc testing cephalothin
Susceptibility disc testing chloramphenicol
Susceptibility disc testing erythromycin
Susceptibility disc testing gentamicins gentamicin
Susceptibility disc testing kanamycin
Susceptibility disc testing novobiocin
Susceptibility disc testing tetracycline
Susceptibility testing
CAMP test for Listeria monocytogenes
Quality control strain for Abbott, and Autobac products


Incorrectly cited in previous editions of this catalog as identical to NCTC 6571.

Handling information

Handling procedure
  1. Open vial according to enclosed instructions. 
  2. Using a single tube of #18 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette.  Rehydrate the entire pellet.
  3. Aseptically transfer this aliquot back into the broth tube.  Mix well.
  4. Use several drops of the suspension to inoculate a #18 agar slant and/or plate.
  5. Incubate the tubes and plate at 37°C for 24 hours.
Handling notes
It is advisable to transfer from agar to agar to maintain MIC values.
Additional information on this culture is available on the ATCC® web site at

Quality control specifications

Verification method
Whole-genome Sequencing


Deposited as
Staphylococcus aureus Rosenbach
Chain of custody
ATCC <-- FDA <-- F Schoenknecht
Cross references
GenBank AX110511 Sequence 1244 from Patent WO0123604.
GenBank AX110995 Sequence 1728 from Patent WO0123604.
GenBank Z16422 S.aureus dfrB gene for dihydrofolate reductase.
GenBank U39769 Staphylococcus aureus 16S-23S ribosomal RNA spacer region.
GenBank U02910 Staphylococcus aureus ATCC 25923 16S rRNA gene, partial sequence.
GenBank AF053568 Staphylococcus aureus ATCC 25923 heat shock protein 60 gene, partial cds.
GenBank AB047239 Staphylococcus aureus DNA, complete structure of cassette chromosome(SCC)-like element, strain:ATCC25923.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

Permits & Restrictions

United States Veterinary Permit for Importation and Transportation of Controlled Materials and Organisms and Vectors

For every order of this item, you must provide a valid Permit for Importation and Transportation of Controlled Materials and Organisms and Vectors (VS Form 16-6A) obtained from the United States Department of Agriculture (USDA), Animal and Plant Health Inspection Service. We cannot ship this item until we receive this permit.

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.


Frequently Asked Questions


Curated Citations

BD MAX™ MRSA Assay. BD Becton Dickinson.

Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard - 9th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M2-A9.

Protocols for Evaluating Dehydrated Mueller-Hinton Agar; Approved Standard. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M6-A2.

Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard - 7th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M7-A8.

Methodology for the Serum Bactericidal test: Approved Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M21-A.

View All Curated Citations for this Product

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