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Thermus aquaticus Brock and Freeze


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Product type
Strain designation
YT-1 [DSM 625, JCM 10724, LMG 8924, NCAIM B.01703, NCIB 11243]
Type strain
Genome sequenced strain
Isolation source
Hot spring at Yellowstone National Park
Geographical isolation
United States; Wyoming
Product format
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information


Specific applications
Produces restriction endonuclease TaqI
Produces restriction endonuclease TaqII


Extremophile type

Handling information

Handling procedure
1. Open vial according to enclosed instructions.

2. Using a single tube of #461 broth (5 to 6 ml), withdraw approximately 0.6 ml with a Pasteur or 1.0 ml pipette.  Rehydrate the entire pellet.

3. Aseptically transfer 0.3 ml of this suspension back into the broth tube.  Mix well.

4. Use the remaining 0.3 ml of the suspension to inoculate a slant of #461 agar.

5. Incubate tubes at 70oC.  For best results, incubate tubes within a closed jar with a moistened paper towel added to maintain humidity and prevent desiccation.

6. Once growth is obtained in broth, plates can be inoculated with approximately 0.2 ml per plate.  Better growth is achieved on plates when incubated at 60oC rather than 70oC.  Plates should be incubated in a jar as described above or taped shut to prevent desiccation.

Handling notes

Yellow growth should be visible in broth and slant tubes after 24 to 48 hours of incubation.  At 1000X magnification, cells are long, regular rods occurring singly or occasionally as end-to-end pairs.

After 48 hours, the colonies on plates are circular with a convex elevation, entire margin, and a smooth surface.  They are dark yellow in color and can be up to 1 mm in diameter.

Quality control specifications

Verification method
Whole-genome Sequencing


Deposited as
Thermus aquaticus Brock and Freeze
TD Brock
Type of isolate
Special collection
National Park Service (NPS) Special Collection
Cross references
GenBank Z15006 T.aquaticus RNase P RNA gene.
GenBank E07542 Gene coding thermotolerance-donating protein.
GenBank J04639 Thermus aquaticus DNA polymerase (PolI) gene.
GenBank E02607 DNA encoding thermostability aminopeptidase(AP-T).
GenBank E07541 Gene coding thermotolerance-donating protein gDNA.
GenBank D00814 Thermus aquaticus gene for aminopeptidase T, complete cds.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

Permits & Restrictions

Material Transfer Agreement Addendum for National Park Service Products

For every order of this item, you must provide a signed Material Transfer Agreement Addendum for National Park Service Products. We cannot ship this item until we receive this addendum. The person signing the addendum as the principal investigator must match the end user as listed on the applicable sales order for the item. Email the signed addendum to [email protected] with a reference to both your account and sales order numbers. Once received, your addendum will be reviewed, and this item will be released for shipment if all requirements are met. If you need assistance with your order, please contact our Customer Care team or your applicable distributor.

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.


Frequently Asked Questions


Curated Citations

Brock TD, Freeze H. Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J. Bacteriol. 98: 289-297, 1969. PubMed: 5781580

Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.

Roberts RJ. Restriction and modification enzymes and their recognition sequences. Nucleic Acids Res. 13: r165-r200, 1985. PubMed: 2987885

type strain

hot spring, Yellowstone National Park

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