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Mycoplasma orale

23714

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Type strain.
Product category
Bacteria
Strain designation
CH 19299 [NCTC 10112]
Type strain
Yes
Genome sequenced strain
Yes
Isolation source
Oropharynx
Geographical isolation
United States; Washington, DC
Applications
Media testing
Quality control
Pharmaceutical testing
Product format
Freeze-dried
Storage conditions
2°C to 8°C

Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
Control strain
Media testing
Pharmaceutical and Personal Care
Recommended as a test microorganism for EP 2.6.7 Mycoplasmas
Recommended as a test microorganism for JP 16 G3 Biotechnological/Biological Products: Mycoplasma Testing for Cell Substrates Used for the Production of Biotechnological/Biological Products
Recommended as a test microorganism for USP <63> Mycoplasma Tests
Preceptrol
No

Handling information

Medium
Temperature
37°C
Atmosphere
Anaerobic
Handling procedure
  1. Follow instructions as suggested for the culturing of Mollicutes:

  2. PROCEDURES FOR PROPAGATING MOLLICUTES:
    1. Open the vial according to the enclosed instructions.
    2. Using a Pasteur or 1.0 mL pipette, withdraw approximately 0.5 to 1.0 mL from a tube containing 2.25 mL. Rehydrate the entire pellet. 
    3. Aseptically transfer this aliquot back into the tube. Mix well.
    4. Make serial dilutions by transferring 0.25 mL from the original tube to a tube containing 2.5 mL. Repeat process by transferring 0.25 mL from the second to a third tube, etc. Dilutions are important, not only for titration purposes, but also to keep culture in varying stages of growth. Many strains will die out rapidly once acid or alkaline conditions are reached. It is recommended to prepare several dilutions from the initial tube as the cryoprotectant used in the freeze drying process often inhibits growth.
    5. Plates may be inoculated to check colony morphology. You can also spot each dilution on the surface of plate (4 or more/plate) to determine the number of colony forming units. However, not all strains do well on solid medium.
    6. Incubate all tubes and plates under the recommended conditions and appropriate temperature. The time necessary for growth will vary from strain to strain. Growth on plates generally requires additional incubation. Depending on the medium used, growth will be indicated by increased turbidity, a color change,or both.

  3. Tubes and plates are incubated under anaerobic conditions using an anaerobe jar or other appropriate method. The incubation temperature is 37°C.
  4. Medium to light turbidity will appear in the first few dilution tubes within 72 hour to 7 days. Colonies generally appear on solid medium within 7 days.
  5. Subsequent, fresh transfer will grow more rapidly than the original rehydrated culture; but usually still requires 48 hours between transfers.
Handling notes
Purified genomic DNA of this strain is available as ATCC® 23714D™.
Additional information on this culture is available on the ATCC® web site at www.atcc.org.

Quality control specifications

Verification method
Whole-genome Sequencing

History

Deposited as
Mycoplasma orale Taylor-Robinson et al.
Depositors
RG Wittler
Chain of custody
ATCC <-- RG Wittler <-- R Purcell <-- D Taylor-Robinson CH 19299 <-- J. Canchola
Type of isolate
Human
Patient age
Child
Cross references
GenBank M24659 M. orale ribosomal RNA small subunit.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

References

Curated Citations

Edward DG, Freundt EA. Type strains of species of the order Mycoplasmatales, including designation of neotypes for Mycoplasma mycoides subsp. mycoides, Mycoplasma agalactiae subsp. agalactiae, and Mycoplasma arthritidis. Int. J. Syst. Bacteriol. 23: 55-61, 1973.

Taylor-Robinson D, et al. A newly identified oral mycoplasma (M. orale) and its relationship to other human mycoplasmas. Am. J. Hyg. 80: 135-148, 1964. PubMed: 14192765

British Pharmacopoeia Commission Tests for microbial contamination. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI B, 2003

. The Mycoplasmatales and the L-phase of bacteria. New York: Appleton-Century-Crofts; 1969.

Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.

View All Curated Citations for this Product

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