Saccharopolyspora hirsuta subsp. kobensis (Iwasaki et al.) Lacey
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
2. Using a single tube of #1877 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #527 agar slant and/or plate.
5. Incubate the tubes and plate at 37°C for 2-3 days.
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Mori T, et al. Antibiotics of the KA-6606 series and pharmaceutical compositions thereof. US Patent 4,206,206 dated Jun 3 1980
Validation list no. 31. Int. J. Syst. Bacteriol. 39: 495-497, 1989.
Bergey's Manual Syst. Bacteriol. 4: 2385-2386, 1989.
Deushi T, et al. A new broad-spectrum aminoglycoside antibiotic complex, sporaricin. I. Fermentation, isolation and characterization. J. Antibiot. 32: 173-179, 1979. PubMed: 37226
Iwasaki A, et al. A new broad-spectrum aminoglycoside antibiotic complex, sporaricin. II. Taxonomic studies on the sporaricin producing strain Saccharopolyspora hirsuta subsp. Kobensis nov. subsp.. J. Antibiot. 32: 180-186, 1979. PubMed: 457579